Western blotting analysis was conducted as previously described [65 (link)]. Primary antibodies against cGAS (Mouse, 1:1000, sc-515777, Santa Cruz Biotechnology) and, GAPDH (GAPDH, A01020, Abbkine, Redlands, CA, USA) were used. ImageJ software (NIH) was used to analyze protein expression levels which were normalized to GAPDH.
Sc 515777
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-515777 is a laboratory instrument designed for sample analysis. It is capable of performing various analytical tasks, but a detailed description of its core function cannot be provided in an unbiased and factual manner without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
A01020, by Abbkine (1 mentions)
Gapdh, by Abbkine (1 mentions)
A1si confocal microscope, by Nikon (1 mentions)
Af3628, by R&D Systems (1 mentions)
Ab33451, by Abcam (1 mentions)
Gb13044, by Wuhan Servicebio Technology (1 mentions)
Nis elements ar imaging software 4, by Nikon (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
2 protocols using sc 515777
1
Western Blot Analysis of cGAS and GAPDH
2
Immunofluorescent Staining of Immune and Vascular Markers
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Immunofluorescent staining was performed as previously described [65 (link)]. The primary antibodies used were as follows: cGAS (Mouse, 1:100, sc-515777, Santa Cruz Biotechnology, Santa Cruz, CA, USA), MOMA-2 (Rat, 1:100, ab33451, Abcam, Cambridge, UK), CD31 (1:100, AF3628, R&D Systems, Minneapolis, MN, USA) and α-SMA (Rabbit, 1:100, GB13044, Servicebio, Wuhan, China). 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, Carlsbad, CA, USA) was applied to stain nuclei. Fluorescent images were captured with a Nikon A1Si confocal microscope (Nikon, Tokyo, Japan), and analyzed with NIS Elements AR Imaging Software 4.10 (Nikon) and ImageJ 1.41 software (NIH, Bethesda, MD, USA).
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