N dicyclohexylcarbodiimide

Alternariol (AOH), Alternariol monomethyl ether (AME), altenuene (ALT), tenuazonic acid (TeA), N, N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), and gold chloride (HAuCl₄·3H₂O) were purchased from Sigma-Aldrich (Shanghai, China). Bovine serum albumin (BSA), human serum albumin (HSA), methyl 4-bromobutanoate, trisodium citrate dihydrate, and Tween-20 were purchased from Amresco (CochranSolon, OH, USA). PEG20000 was purchased from Alfa Aesar Chemical Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) were purchased from Thermo Fisher Scientific (Waltham, NJ, USA). Incomplete Freund’s adjuvant, complete Freund’s adjuvant, 96-well microplates, goat anti-mouse IgG, and developing solution was offered by Beijing Kwinbon Biotechnology Co., Ltd. (Beijing, China). Polyvinyl chloride (PVC) sheet, NC membrane (Millipore 135), absorbent pad, conjugate pad, and sample pad were obtained from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). Analytical grade sodium chloride (NaCl), dimethylformamide (DMF), methyl 4-bromobutanoate, potassium carbonate (K₂CO₃), ethanol, petroleum ether, tetrahydrofuran (THF), lithium hydroxide (LiOH), ethyl acetate (EtOAc), and other chemicals and solvents were ordered from Beijing Chemical Reagent Co. (Beijing, China). Water was deionized and purified with a Milli-Q system (Millipore, Bedford, MA, USA).

Different reagents were used throughout the research project. All the reagents used were of analytical grade and were purchased from reliable resources, and these reagents include the following: benzoyl chloride, triethylamine, and N, N′-dicyclohexylcarbodiimide (DCC) were purchased form (Sigma-Aldrich, the United States. 4-Dimethylaminopyridine (DMAP) was purchased from Merck, Germany. Benzoic acid was purchased from Alfa Aesar, the United Kingdom. Dichloromethane (DCM), methanol, ammonium chloride, magnesium sulfate, and acetonitrile (ACN) were purchased from Sigma-Aldrich, Germany. Resorcinol, catechol, and hydroquinol were purchased from Sigma-Aldrich, the UK. Gabapentin was donated as a gift from Birzeit Pharmaceutical Company, Palestine.