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Mouse anti e cadherin and n cadhherin polyclonal antibodies

Manufactured by BD

Mouse anti-E-cadherin and -N-cadherin polyclonal antibodies are laboratory reagents used for the detection and analysis of E-cadherin and N-cadherin proteins in biological samples. These antibodies are produced in mice and are designed to recognize and bind to specific epitopes on the target proteins.

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2 protocols using mouse anti e cadherin and n cadhherin polyclonal antibodies

1

Immunohistochemical Analysis of Cadherins

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Cells were fixed in 4% paraformaldehyde following a standard protocol. Mouse anti-E-cadherin and -N-cadhherin polyclonal antibodies (1:100; BD) were used as primary antibodies, with TRITC-labeled anti-Rabbit IgG (1:200; Sigma) used as a secondary antibody. Sections were mounted onto slides using Gel Mount Aqueous Mounting Medium (G0918, Sigma) and examined with an OlympusBX51 microscope (Olympus Optical, Tokyo, Japan).
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2

Immunofluorescence Imaging of E-cadherin and N-cadherin

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Cells were fixed in 4% paraformaldehyde following a standard protocol. Mouse anti-E-cadherin and -N-cadhherin polyclonal antibodies (1:100; BD) were used as primary antibodies, with TRITC-labeled anti-Rabbit IgG (1:200; Sigma) used as a secondary antibody. Sections were mounted onto slides using Gel Mount Aqueous Mounting Medium (G0918, Sigma) and examined with an Olympus BX51 microscope (Olympus Optical, Tokyo, Japan).
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