Penicillin g

Antibiogram tests were performed to screen resistance or sensitivity of AGA58 against commonly used antibiotics. Commercially available antibiotic disks [methicillin, vancomycin, amikacin, kanamycin, azithromycin, tetracycline, penicillin G (Bioanalyse, Yenimahalle, Ankara, Turkey); ampicillin, oxacillin, carbenicillin, amoxicillin, streptomycin, erythromycin, rifampicin (Oxoid, Basingstoke, Hampshire, UK)] were utilized to determine antibiotic susceptibility of L. nagelii AGA58 . The disk diffusion assay was performed per modified Kirby-Bauer method [29] (link). Interpretation of inhibition zone (mm) results was performed per Clinical and Laboratory Standards Institute's performance standards for antimicrobial testing [30] .
Inhibition zone less than or equal to 14 mm was noted resistant (R). Inhibition zone greater than 20 mm was regarded sensitive (S). Zones between 15 and 19mm were recorded as semi-sensitive or intermediate (I).

Antibiogram assays were conducted to nd out the resistance or sensitivity of the DY46 strain against commonly used antibiotics. Ready to use commercial antibiotic disks (methicillin, vancomycin, amikacin, kanamycin, azithromycin, tetracycline, penicillin G (Bioanalyse); ampicillin, oxacillin, carbenicillin, amoxycillin, streptomycin, erytromycin, rifampacin (oxoid)) were employed for antibiotic susceptibility testing of L. plantarum DY46. The application of disk diffusion assay was performed according to a modi ed Kirby-Bauer method (Sharma et al. 2017 (link)). Interpretation of inhibition zone (mm) results was carried out with regard to Clinical and Laboratory Standards Institute's performance standards for antimicrobial testing (CLSI M100-S22, 2012). Results with an inhibition zone less than or equal to 14mm were noted resistant (R). Additionally, inhibition zones greater than 20 mm were considered sensitive (S) and between 15-19 mm were accepted as semi-sensitive or intermediate (I).