Cd11a

Poly(D,L-lactide-co-glycolide) (PLGA), (PLA:PGA 75:25, Mw = 66,000-107,000) was purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). It was used for the fabrication of fibrous PLGA scaffolds by using dichloromethane from Merck Millipore (Burlington, MA, USA), as a solvent via the wet spinning technique. In this process, the mixture of isopropanol from Merck Millipore and distilled water (IP:DW) was also used in different ratios as a coagulation bath to obtain PLGA precipitate. In in vitro studies, alpha MEM medium (Gibco, Invitrogen, USA) supplied with 1% penicillin-streptomycin, and 10% fetal bovine serum (FBS) (Gibco, Invitrogen, USA) was used. For the characterization of isolated rBMSCs, CD29 (BD Pharmingen, USA), CD45 (BD Pharmingen, USA), CD11a (Abcam, USA), CD90 (BD Pharmingen, USA), and CD31 (Abcam, USA) antibodies were used via flow cytometry analysis. Cell proliferation on the fabricated PLGA scaffolds was analyzed with MTS reagent (Promega, USA).

The rabbit polyclonal anti-cGAS K491me antibody was produced by Boer Biotechnology (Chengdu, China). The mouse cGAS K491me peptide was synthesized and injected into rabbits. An affinity column with bound nonmodified peptide was used to collect and purify rabbit serum, excluding antibodies targeting nonmethylated cGAS. The antibody was then purified using an affinity column linked with bound cGAS K491me peptide. A rabbit polyclonal antibody recognizing human cGAS K506me was produced following the same protocol.
Antibodies recognizing cGAS, Tubulin, GST, His, SET1A, SET1B, SET7, MLL1, MLL3, γH2A.X and SMYD3 were obtained from Cell Signaling Technology. Antibodies recognizing RIOX2 were purchased from Atlas Antibodies. Antibodies recognizing RIOX1, ASH1L, PARP1, MLL2, SGF29, Timeless, NSD1, NSD2, H2A.X, CD11b, CD11a, CD34, Thy-1, and SMYD2 were purchased from Abcam. The antibody against Flag, anti-Flag M2 agarose beads, streptavidin-conjugated agarose beads, biotin, bovine serum albumin and poly(ADP-ribose) glycohydrolase (PARG) recombinant protein were purchased from Sigma. Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were purchased from Thermo Fisher Scientific. biotin (terminal)-PAR Polymer was purchased from Trevigen.

MMP Inhibitor V and MMP Inhibitor Set I were purchased from Calbiochem (San Diego, CA, USA). GI254023X was purchased from Sigma‐Aldrich (St Louis, MO, USA). rh‐TNF‐α and rh‐WNT3A were purchased from R&D (Minneapolis, MN, USA), and rh‐sICAM‐1 was purchased from eBioscience (Waltham, MA, USA.). rhMMP‐9 and an antibody against WNT3A were purchased from Millipore (Billerica, MA, USA). Antibodies against HA‐tag, FN, ICAM‐1, anti‐mouse IgG‐HRP, anti‐goat IgG‐HRP, and anti‐rabbit IgG‐HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies to anti‐goat Alexa Fluor 488, anti‐mouse Alexa Fluor 546 were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against CD31, VIM, YKL‐40, p‐Src (Tyr418), IBA‐1, TNF‐α, CD11a, and ICAM‐1 were purchased from Abcam (Cambridge, UK). Antibodies against p‐PKCδ (Tyr311) and p‐STAT3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against IBA‐1 (Novus Biologicals, Littleton, CO, USA), CD11b (BioLegend, San Diego, CA, USA), and N‐cadherin (BD Biosciences, San Jose, CA, USA) were also used in this study. Antibodies against β‐actin and ZEB1 were obtained from Sigma‐Aldrich.