Dynabeads untouched human cd4 or cd8 t cells kit

Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donor’s buffy coats obtained from Rigshospitalet, Copenhagen, Denmark in accordance with the Declaration of Helsinki. Written informed consent was obtained from blood donors at the Department of Clinical Immunology, University Hospital Rigshospitalet, Copenhagen and used without the possibility to identify case specific information. The ethical committee, Region H, Capital Region of Denmark, approved the use of these buffy coats for research that was carried out in accordance with the approved guidelines. Lymphoprep density-gradient centrifugation (Stemcell technologies) was used and the isolated cells taken for the following steps, as needed. CD4⁺ T cells and CD8⁺ T cells were separately enriched using Dynabeads Untouched Human CD4⁺ or CD8⁺ T cells kit (Invitrogen, cat. 11346D or 11348D, respectively) following the manufacturer’s instructions. When specified, T cells were stimulated in vitro with Dynabeads Human T activator CD3/CD28 for T cells expansion and activation (Thermo Fisher, cat # 11131D), (5:2 cell:beads ratio).