H1 hESCs (WA-01) were maintained in Essential 8 FLEX medium (Thermo Fisher) on cell culture dishes coated with recombinant Vitronectin (Thermo Fisher). Human patient-derived cell lines (HSJD002 and SJHGGx6) were maintained in a 1:1 mixture of DMEM/F12 medium and Neurobasal medium (Thermo Fisher) supplemented with B27 without Vitamin A (Thermo Fisher), EGF (20 ng/ml), basic FGF (20 ng/ml, R&D Systems), PDGF-AA and -BB (20 ng/ml, Peprotech) and heparin (10 ng/ml, Stemcell Technologies). HEK293T cells were maintained in DMEM medium (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS).
Recombinant vitronectin
Manufactured by Thermo Fisher Scientific
Recombinant Vitronectin is a purified glycoprotein derived from recombinant DNA technology. It serves as an extracellular matrix protein that promotes cell attachment and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Basic fgf, by R&D Systems (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
B27 without vitamin a, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by R&D Systems (1 mentions)
Essential 8 flex medium, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Heparin, by STEMCELL (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Advanced dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Cc 2509, by Lonza (1 mentions)
Cc 2511, by Lonza (1 mentions)
2 protocols using recombinant vitronectin
1
Maintenance of hESCs, Patient-Derived Cell Lines, and HEK293T Cells
2
Generating iPSCs from Healthy and SAA Fibroblasts
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Human fibroblasts from three healthy volunteers, one neonatal (Lonza, CC-2509) and two adult (Lonza, CC-2511), and three patients with pediatric SAA were cultured with Advanced Dulbecco’s Modified Eagle Medium (Thermo-Fisher, Waltham, MA, USA) containing 10% FBS (Thermo Fisher Scientific), 1% Glutamax (Thermo Fisher Scientific) and 1% penicillin/streptomycin (Thermo Fisher Scientific) at 37 °C and 5% CO2 in a humidified incubator. Fibroblasts were transduced using the CytotuneTM-iPS Reprogramming Kit (Thermo Fisher Scientific, A13780-01) according to manufacturer’s instructions. iPSC colonies were established on inactivated primary mouse embryonic fibroblasts feeder layer and then adapted to a feeder-free system, cultured on recombinant Vitronectin (Thermo Fisher Scientific) and in StemPro hESC SFM® media (Thermo Fisher Scientific) supplemented with 8ng/ml basic Fibroblast Growth Factor (Thermo Fisher Scientific), 1% penicillin/streptomycin and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific).
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