HK‐2 cells were seeded in 6‐well plates (1.5 × 106 cells/well) and incubated for 24 hours. Next, the medium was replaced with DMEM/F12 with or without COM (300 µg/mL) and incubate for 48 hours. All cells in each well were collected and doubly stained with Annexin V‐FITC/propidium iodide (PI) or Annexin V‐APC/7‐ADD Apoptosis Detection kit (BD Biosciences) according to the manufacturer's instructions. Finally, the proportions of cell apoptosis were determined by flow cytometry (BD FACSCalibur). Three independent experiments were conducted in triplicate.
Annexin 5 apc 7 add apoptosis detection kit
Manufactured by BD
Sourced in United States
The Annexin V-APC/7-ADD Apoptosis Detection kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and 7-amino-actinomycin D (7-ADD), a DNA-binding dye, to identify cells undergoing apoptosis.
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2 protocols using annexin 5 apc 7 add apoptosis detection kit
1
Apoptosis Analysis of HK-2 Cells
2
Annexin V-APC/7-AAD Apoptosis Assay
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Apoptotic cells were detected using an Annexin V-APC/7-ADD apoptosis detection kit (BD Biosciences, USA). Briefly, cells were detached using EDTA-free trypsin, centrifuged, and the supernatant discarded. They were then rinsed with ice-cold PBS, centrifuged, and the cell pellet resuspended in 200 μl of 1 × binding buffer. The cells were then transferred into a FACS tube and stained with Annexin V-APC and 7-AAD (5µL each) in the dark for 15 mins, at room temperature. Next, flow cytometry measurements were taken, and the data analyzed on FlowJo 8.1.
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