Tr400pb

Manufactured by Oxford Instruments

The TR400PB is a laboratory equipment product designed for accurate temperature measurement. It features a high-precision thermometer with a wide temperature range. The core function of the TR400PB is to provide reliable and accurate temperature readings for various laboratory applications.

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Lab products found in correlation

Mfp 3d spm, by Oxford Instruments (1 mentions) Asyelec 01 r2, by Oxford Instruments (1 mentions)

2 protocols using tr400pb

Nuclear Stiffness Measurement via AFM

Cited 1 time

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Samples were probed with Asylum-MFP3D-Bio-AFM mounted on an Olympus IX-71 inverted optical microscope platform. Stiff pyramidal cantilevers with a nominal spring constant of ~10 pN/nm tips (32 kHz; TR400PB; Asylum Research) were used to measure nuclear stiffness. Indentation data of 500-2,000 nm were fitted to nuclear stiffness measurements using the Hertz model to obtain estimates of nuclear stiffness 5 (link), 31 (link).

Li Y., Li Q., Mu L., Hu Y., Yan C., Zhao H., Mi Y., Li X., Tao D, & Qin J. (2024). Nuclear Softness Promotes the Metastatic Potential of Large-Nucleated Colorectal Cancer Cells via the ErbB4-Akt1-Lamin A/C Signaling Pathway. International Journal of Biological Sciences, 20(7), 2748-2762.

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Atomic Force Microscopy Techniques Comparison

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All atomic force microscopy (AFM), scanning Kelvin probe force microscopy (sKPFM), piezo force microscopy (PFM), and amplitude modulated force microscopy (AMFM) experiments were carried out on an Asylum Research model MFP-3D SPM. PFM experiments were conducted using dual-AC resonance tracking (DART-PFM) mode. Three sets of cantilevers consisting of six individual probes of varying spring constants were used: ASYELEC-01 R2 (R2), Asylum Research, are iridium-coated conductive silicon probes with a 70.0 ± 19.5 kHz free air resonance frequency, and a ∼280 kHz contact resonance. The R2 has a free air spring constant of 2.8 ± 1.4 N m⁻¹. HQ: NSC36/PT (NSC: A, B, and C), MikroMasch, are platinum-coated conductive silicon probes with three independent levers per chip. The levers have a 90 ± 65, 130 ± 98, 65 ± 45 kHz free air resonance frequency for levers A, B and C respectively, giving a ∼340, 520, and 260 kHz contact resonance for each lever. The NSC levers have a free air spring constant of 1.0 ± 2, 2.0 ± 4.5, and 0.6 ± 1.25 N m⁻¹. TR400PB (TR: S and L), Asylum Research, are gold-coated conductive silicon nitride probes with a 32.0 ± 14.5 and 10.0 ± 7 kHz free air resonance frequency, but a ∼120 and 40 kHz contact resonance. The TR levers have a free air spring constant of 0.09 ± 0.12 and 0.02 ± 0.02 N m⁻¹ respectively.

Miller N.C., Grimm H.M., Horne W.S, & Hutchison G.R. (2019). Accurate electromechanical characterization of soft molecular monolayers using piezo force microscopy. Nanoscale Advances, 1(12), 4834-4843.

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