SA-β-Gal staining was performed using a Cell senescence β-galactosidase staining kit (Solarbio, G1580, Beijing, China) according to the manufacturer’s protocol. Briefly, cells were washed with PBS and fixed for 15 min. Then, the cells were washed with PBS and incubated with SA-β-Gal staining solution for 16 h at 37 °C. SA-β-Gal-positive cells were calculated in three random fields per culture dish and analyzed using ImageJ (NIH, Bethesda, MD, USA) and senescence was scored as a percentage of SA-β-Gal-positive cells relative to the total cell number.
Cell senescence β galactosidase staining kit
Manufactured by Solarbio
Sourced in China
The Cell senescence β-galactosidase staining kit is a laboratory tool used to detect and quantify the presence of senescent cells. It measures the activity of the lysosomal enzyme β-galactosidase, which is a widely used biomarker for cellular senescence.
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2 protocols using cell senescence β galactosidase staining kit
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Senescence-Associated β-Galactosidase Assay
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Senescence-Associated β-Galactosidase Assay
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SA-β-Gal staining was performed using a Cell senescence β-galactosidase staining kit (Solarbio, G1580, Beijing, China) according to the manufacturer’s protocol. Briefly, cells were washed with PBS and fixed for 15 min. Then, the cells were washed with PBS and incubated with SA-β-Gal staining solution for 16 h at 37 °C. SA-β-Gal-positive cells were calculated in three random fields per culture dish and analyzed using ImageJ (NIH, Bethesda, MD, USA) and senescence was scored as a percentage of SA-β-Gal-positive cells relative to the total cell number.
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