Hydragel agarose gels

Manufactured by Sebia

Hydragel agarose gels are laboratory products used for the separation and analysis of biomolecules, such as proteins, enzymes, and nucleic acids, through electrophoresis. They provide a stable and consistent matrix for the electrophoretic separation process.

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Lab products found in correlation

Hydrasys densitometer, by Sebia (2 mentions) Phoresis software, by Sebia (2 mentions) Hydrasys, by Sebia (2 mentions)

Spelling variants of this product

Sebia Hydragel 15/30 lipoprotein (e) agarose gels (3 citations)

2 protocols using hydragel agarose gels

Serum Protein Electrophoresis Profiling

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Blood was collected in Eppendorf by retro-orbital sampling. Semiautomated electrophoresis was performed on the Hydrasys instrument (Sebia, Lissex, France). According to the manufacturer’s instructions, 10 μl of undiluted serum were manually applied to the Hydragel agarose gels (Sebia). The subsequent steps, electrophoresis (pH 9.2, 20 W constant current at 20°C), drying, amidoblack staining, de-staining, and final drying, were carried out automatically. The use of Hydrasys densitometer and Phoresis software (Sebia) for scanning resulting profiles provided accurate relative concentrations (percentage) of individual protein zones. M-spike levels were calculated as total gamma globulins/albumin ratio (G/A).

Valtorta S., Toscani D., Chiu M., Sartori A., Coliva A., Brevi A., Taurino G., Grioni M., Ruffini L., Vacondio F., Zanardi F., Bellone M., Moresco R.M., Bussolati O, & Giuliani N. (2021). [18F](2S,4R)-4-Fluoroglutamine as a New Positron Emission Tomography Tracer in Myeloma. Frontiers in Oncology, 11, 760732.

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Serum Protein Electrophoresis in Mice

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Mouse blood was periodically collected in Eppendorf by retro-orbital sampling. Semi-automated electrophoresis was performed on the Hydrasys instrument (Sebia, Lissex, France). According to the manufacturer’s instructions, 10 µL of undiluted serum were manually applied to the Hydragel agarose gels (Sebia). The subsequent steps: electrophoresis (pH 9.2, 20 W constant current at 20 °C), drying, amidoblack staining, de-staining and final drying were carried out automatically. The use of Hydrasys densitometer and Phoresis software (Sebia) for scanning resulting profiles provided accurate relative concentrations (percentage) of individual protein zones. M-spike levels were calculated as total gamma globulins/albumin ratio (G/A)^{17 (link)}.

Calcinotto A., Brevi A., Chesi M., Ferrarese R., Garcia Perez L., Grioni M., Kumar S., Garbitt V.M., Sharik M.E., Henderson K.J., Tonon G., Tomura M., Miwa Y., Esplugues E., Flavell R.A., Huber S., Canducci F., Rajkumar V.S., Bergsagel P.L, & Bellone M. (2018). Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression. Nature Communications, 9, 4832.

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