HepG2 and SMMC-7721 cells were purchased from ATCC. HepG2 cells were cultured in DMEM high glucose medium (Servicebio), and SMMC-7721 cells were cultured in 1640 medium (Servicebio), all containing 10% Foetal Bovine Serum (FBS) and 1% penicillin and streptomycin (Servicebio). Short hairpin (sh)RNAs sh-STK25 were obtained from miaolingbio.lnc, Wuhan, China. HepG2 and SMMC-7721 cells were cultured in 6-well plates (5 × 105/well) and transfected with 2.4 μg siRNA using Attractence Transfection Reagent (Cat. No. 301005, QIAGEN, China).
Foetal bovine serum (fbs)
Manufactured by Wuhan Servicebio Technology
Sourced in China
Foetal Bovine Serum is a commonly used cell culture supplement derived from the blood of bovine fetuses. It provides a complex mixture of proteins, growth factors, and other components that support the growth and proliferation of cells in in vitro cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Multiskan sky, by Thermo Fisher Scientific (3 mentions)
Penicillin and streptomycin, by Wuhan Servicebio Technology (1 mentions)
1640 medium, by Wuhan Servicebio Technology (1 mentions)
Hepg2, by Wuhan Servicebio Technology (1 mentions)
Dmem high glucose medium, by Wuhan Servicebio Technology (1 mentions)
Smmc 7721, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Wuhan Servicebio Technology (1 mentions)
Mtt solution, by Wuhan Servicebio Technology (1 mentions)
2 protocols using foetal bovine serum (fbs)
1
Silencing STK25 in HepG2 and SMMC-7721 Cells
2
Cell Viability Assay Using MTT
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell lines were purchased from Servicebio (Wuhan, China) and cultured in RPMI1640 (Servicebio, Wuhan, China) with 10% foetal bovine serum and 1% antibiotic agent (Servicebio, Wuhan, China) at 37 °C. With the treatment of synthetic compounds, cells were cultured for 48 h and added with MTT solution (Servicebio, Wuhan, China). After 4 h, medium was removed and DMSO was added to culture for 30 min. Finally, the absorbance value was obtained by a microplate reader (Multiskan Sky, Thermo Fisher, Shanghai, China).
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