For immunostaining, we used the Bondmax automated slide staining system (Leica Microsystems, Wetzlar, Germany), a rabbit polyclonal anti-human-MPO antibody (dilution 1:2000; Dako, Carpinteria, CA, USA), and a monoclonal mouse anti-human CD8 antibody (1:100; Dako, Carpinteria, CA, USA) diluted with Bond Primary Antibody Diluent (Leica, Newcastle, UK). Immunostaining was visualized with a Bond Polymer Refine Detection Kit (Leica Biosystems, Newcastle, UK). Bond Epitope Retrieval Solution 2 (Leica Biosystems, Newcastle, UK) was used to carry out automated antigen retrieval at 20 min. The omission of the primary antibody served as negative control.
Monoclonal mouse anti human cd8 antibody
Manufactured by Agilent Technologies
Sourced in United States, Switzerland
The Monoclonal mouse anti-human CD8 antibody is a laboratory reagent used in immunological research and analysis. It specifically binds to the CD8 protein, which is expressed on the surface of certain T cells. This antibody can be used to identify and isolate CD8-positive cells for further study.
Automatically generated - may contain errors
2 protocols using monoclonal mouse anti human cd8 antibody
1
Automated Immunostaining of MPO and CD8
2
Automated Immunohistochemical Staining Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemical stainings of tissue sections were performed using automated immunostainers (Ventana Medical Systems, Tucson, AZ, USA or Leica BOND-III, Leica Microsystems, Heerbrugg, Switzerland) utilizing the antibodies monoclonal rabbit-anti-human CD4+-antibody (Ventana, clone SP35, antibody prediluted), monoclonal mouse-anti-human CD8+-antibody (Dako, Baar, Switzerland, clone C8/144B, antibody dilution: 1:100) and monoclonal mouse-anti-human CD20-antibody (Ventana, clone L26, antibody pre-diluted) with pretreatments according to the respective manufactures' instructions. Antibody detections were performed with either Ultra View-AP Kit (substrate: New-Fuchsin) on Ventana Benchmark or Refine-HRP-Kit on BondMax Benchmark, Leica.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!