B 1 quant ps

¹H NMR spectroscopy measurements were performed according to established protocols.¹⁵ The frozen serum samples were thawed at room temperature for about 30 min before being mixed with phosphate buffer (1:1) to a volume of 600 μL. The resulting mixture was pipetted into the NMR sample tube and immediately prepared for measurement.
All measurements were run on a Bruker 600‐MHz Avance III Neo NMR‐spectrometer equipped with a BBI Probe and a Bruker SampleJet robot with a cooling system for sample storage at 4°C. The samples were measured at 310 K, and a full quantitative calibration was completed before the measurement. All experiments were completed using the Bruker in vitro diagnostics (IVDr) methods.¹⁵ All data were processed in automation using Bruker TopSpin 4.1.1 and ICON‐NMR. Metabolite reports (40 parameters) were obtained using Bruker IVDr B.I. Methods Plasma/Serum Analysis (B.I.Quant‐PS, v.2.0.0). Triglycerides and cholesterol concentration were assessed using Bruker Lipoprotein Subclass Analysis (B.I.LISA, v.1.0.0). The analysis was performed on 1D Nuclear Overhauser Spectroscopy experiments (1D NOESY).