Ab138500

Manufactured by Abcam

Ab138500 is a lab equipment product designed for scientific research purposes. It serves as a core component for various experimental setups. The product specification and technical details are available upon request.

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Lab products found in correlation

2 protocols using ab138500

Antibody Characterization for Cell Biology Research

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Antibodies used in this study include antiactin (MAB1501; EMD Millipore), anti–α-adaptin (sc-17771; Santa Cruz Biotechnology, Inc.), anti-CD63 (556019; BD), anti-DNA (61014; Progen), anti-Drp1 (611113; BD), anti-Eps15 (sc-534; Santa Cruz Biotechnology, Inc.), anti-Flag (anti-DDDDK, ab1257; Abcam), anti-Flag (F1804; Sigma-Aldrich), anti-GFP (ab6673; Abcam), anti-GFP (A6455; Invitrogen), anti-LAMP2 (sc-18822; Santa Cruz Biotechnology, Inc.), anti-LBPA (Z-SLBPA; Eschelon), anti-parkin (sc-32282; Santa Cruz Biotechnology, Inc.), anti-PDH E2/E3bp (ab110333; Abcam), anti-PINK1 (6946; Cell Signaling Technology), anti-PMP70 (SAB4200181; Sigma-Aldrich), anti-SDHA (ab14715; Abcam), anti-SNAP29 (ab138500; Abcam), anti-Stx17 (17815–1-AP; ProteinTech), anti-Stx17 (HPA001204; Sigma-Aldrich), anti-TIM23 (611222; BD), anti-TIP47 (Novus Biologicals), anti-TOM20 (sc-11414; Santa Cruz Biotechnology, Inc.), anti-UQCRFS1 (referred to herein as CIII-Rieske, ab14746; Abcam), anti-VAMP7 (sc-166394; Santa Cruz Biotechnology, Inc.), anti-VAMP8 (ab76021; Abcam), anti-VDAC1 (ab14734; Abcam), and anti-Vps41 (ab181078; Abcam). Unless otherwise specified, all reagents were purchased from Sigma-Aldrich. The TIP47 antibody was a gift from P. McPherson (McGill University, Montreal, Quebec, Canada).

McLelland G.L., Lee S.A., McBride H.M, & Fon E.A. (2016). Syntaxin-17 delivers PINK1/parkin-dependent mitochondrial vesicles to the endolysosomal system. The Journal of Cell Biology, 214(3), 275-291.

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Molecular Markers of Autophagy and Cell Signaling

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We used primary antibodies to the following proteins: LC3B (Cell Signaling Technology, 3868), ATG5 (Cell Signaling Technology, 12994), ATG7 (Cell Signaling Technology, 8558), ATG12 (Cell Signaling Technology, 4180), TUBB/β-Tubulin (Cell Signaling Technology, 2146), p-EIF4EBP1/4E-BP1 (Cell Signaling Technology, 2855), EIF4EBP1/4E-BP1 (Cell Signaling Technology, 9452), RPS6KB/S6K (Cell Signaling Technology, 2708), p-RPS6KB/S6K (Cell Signaling Technology, 9234), p-ULK1 (Ser317; Cell Signaling Technology, 12753), p-ULK1 (Ser757; Cell Signaling Technology, 14202), ULK1 (Cell Signaling Technology, 8054), mCherry (Medical & Biological Laboratories, PM005), GFP (Thermo Fisher Scientific, A11122), FLAG epitope (Sigma-Aldrich, F1804), MYC epitope (Santa Cruz Biotechnology, sc-789), BORCS5 (Abgent, AP5806b), SNAP29 (Abcam, ab138500) and VAMP8 (Abcam, ab76021). HRP-conjugated secondary antibodies were from Perkin Elmer (NEF822001EA, NEF812001EA). Secondary antibodies conjugated to Alexa Fluor dyes were from Thermo Fisher Scientific. Baf (B1793) and Torin2 (SML1224) were from Sigma-Aldrich.

Jia R., Guardia C.M., Pu J., Chen Y, & Bonifacino J.S. (2017). BORC coordinates encounter and fusion of lysosomes with autophagosomes. Autophagy, 13(10), 1648-1663.

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