Nc oligos

Manufactured by GenePharma

Sourced in China

NC) oligos are synthetic DNA or RNA oligonucleotides with a 3' amino-modified group. They are used as building blocks in the synthesis of various biomolecules and for various research and diagnostic applications.

Automatically generated - may contain errors

Lab products found in correlation

Mir 21 mimic, by GenePharma (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions) Pcdh cmv mcs ef1 cogfp construct, by System Biosciences (1 mentions) Pten sirna, by GenePharma (1 mentions) Polybrene, by Merck Group (1 mentions) Sirnas, by RiboBio (1 mentions)

2 protocols using nc oligos

Modulating miR-21 and LINC00312 in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

An miR‐21 mimic, a miR‐21 inhibitor, PTEN siRNA and their respective negative control (NC) oligos were purchased from Genepharma Co., Ltd. (Shanghai, China). Oligonucleotide transfection was performed with Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). The complementary DNA encoding LINC00312 was cloned into the pCDH‐CMV‐MCS‐EF1‐coGFP construct (System Biosciences, CA, USA) to generate the pCDH‐CMV‐LINC00312 expression vector. The production and purification of lentivirus particles were conducted as described by Zheng and colleagues.13 The packaged lentivirus particles were named Lv‐LINC00312. The empty lentiviral vector Lv‐control served as a control. Cells were infected with recombinant lentivirus‐transducing units plus 5 mg/mL Polybrene (Sigma, St. Louis, MO, USA).

Li G., Wang C., Wang Y., Xu B, & Zhang W. (2018). LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21. Journal of Cellular and Molecular Medicine, 22(11), 5565-5572.

+ Open protocol

+ Expand

Transfection of HNSCC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SCC25 or CAL27 cells transfected with negative control (NC) oligos or miR-21 mimics (20 nM; Genepharma, Shanghai, China) were labeled as NC or miR-21, respectively. HNSCC cells transfected with si-NC or siRNAs (20 nM; RIBOBIO, Guangzhou, China) against STAT3 or VHL, labeled as si-NC, si-STAT3, or si-VHL, respectively, using Lipofectamine 2000 reagent according to the manufacturer's instructions (Life Technology, New York, USA). During transfection, Opti-MEM medium without FBS was employed, and the transfected medium was replaced with DMEM-F12 or DMEM after 6 h. The sequences of both the miRNAs and siRNAs are listed in Supplementary Table 1.

Wang Y., Wang S., Wu Y., Ren Y., Li Z., Yao X., Zhang C., Ye N., Jing C., Dong J., Zhang K., Sun S., Zhao M., Guo W., Qu X., Qiao Y., Chen H., Kong L., Jin R., Wang X., Zhang L., Zhou J., Shen Q, & Zhou X. (2017). Suppression of the Growth and Invasion of Human Head and Neck Squamous Cell Carcinomas via Regulating STAT3 Signaling and miR-21/β-catenin Axis with HJC0152. Molecular cancer therapeutics, 16(4), 578-590.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!