Tri 3d bon image analyzer

After the pull-out test, the femur was subjected to microcomputed tomography (mCT) scanning performed with an R_mCT2 device (Rigaku, Tokyo, Japan) using a 90-kV anode electrical current at a 30-μm resolution. The isotropic voxel resolution was 30 μm × 30 μm × 30 μm. To verify new bone formation around the implant, a 1.5-mm² area surrounding the bone where the 1.2-mm implant was placed was scanned from a depth of 0.5–1.0 mm from the inner cortical bone. Thus, a cuboid of peri-implant bone with a 1.5-mm × 1.5-mm base and a 0.5-mm height (Fig. 2) was used to analyze BMD. R_mCT Image Analysis software (Rigaku, Tokyo, Japan) was used to generate three-dimensional models using the scanned data. A TRI/3D-BON image analyzer (Ratoc System Engineering, Tokyo, Japan) was used to calculate the BMD of the peri-implant bone cuboid and generate color images depicting BMD intensity, with blue and light blue, green and yellow, and orange and red representing low, medium, and high BMD values, respectively.

After removal of the implant, the femur was subjected to micro-computed tomography (mCT) scanning. mCT was performed with an R_mCT2 device (Rigaku, Tokyo, Japan) using a 90-kV anode electrical current at a 30-μm resolution. The isotropic voxel resolution was 30 μm × 30 μm × 30 μm. To verify new bone formation around the implant, a 1.5-mm² area surrounding the bone where the 1.2-mm implant had been placed was scanned from a depth of 0.5-mm to 1.0-mm depth from the inner cortical bone. Next, the trabecular structure of the 1.5 mm × 1.5 mm × 0.5 mm volume of peri-implant bone was assessed by the TRI/3D-BON image analyzer (Ratoc System Engineering, Tokyo, Japan) (Fig. 1). Analysis of BS/BV (1/mm), BV/TV (%), TbTh (μm), TbN (1/mm), Vtr (mm³), and micro-CT image was performed for each group containing nine rats (Fig. 2).