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Lh 780 automated hematology analyzer

Manufactured by Beckman Coulter
Sourced in Japan, United States

The LH 780 is an automated hematology analyzer designed for high-volume clinical laboratory testing. The instrument provides complete blood count (CBC) analysis, including the measurement of key parameters such as red blood cells, white blood cells, and platelets. The LH 780 utilizes advanced technologies to deliver reliable and accurate results, supporting the needs of healthcare professionals in diagnostic and research settings.

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2 protocols using lh 780 automated hematology analyzer

1

Diagnostic Criteria for Hereditary Spherocytosis

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All probands were diagnosed with HS according to the ICSH guidelines [12 (link)]. Intravenous blood samples were collected (with one tube for EDTA-K2 anticoagulation and one tube for separation gel coagulation). Liver function, especially total bilirubin (TBIL) and direct bilirubin (DBIL), was performed using a 7600 full automatic biochemical instrument (Hitachi, Tokyo, Japan), and an LH 780 automated hematology analyzer (Beckman Coulter, California, USA) was used for blood routine examination. Spherocytosis in the peripheral blood was stained with Wright's staining, and the cells' morphological examination and number counting were rechecked with an ordinary microscope. Five hundred red blood cells were evaluated in each blood smear for the percentage of spherical red cells, and red cells with other shape abnormalities. The guidelines for the standardization of peripheral blood smear interpretation were from the Chinese Journal of Laboratory Diagnosis.
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2

Hematological and Liver Function Assessment

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Written informed consent was obtained from all participants enrolled in the present study. The study protocol was approved by the Ethics Committee of The First Affiliated Hospital at the Guangxi Medical University (Nanning, China). A total of 7 ml of blood was collected from the proband and 95 healthy volunteers (2 ml of blood was used for EDTA anticoagulation and 5 ml was used for separation of coagulation factors). The proband (3 year old female) was recruited following routine surgery at the Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, and all participants (57 males and 38 females; mean age of 23.15±11.59 years; age range of 7–51 years) in the present study were recruited between January 2016 and December 2016 at The First Affiliated Hospital of Guangxi Medical University.
Blood tests, including RBC count, hemoglobin (HGB) levels, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean sphered blood cell volume (MSCV), mean reticulocyte volume, reticulocyte (RET) levels and mean platelet volume (MPV), were performed using the LH 780 automated hematology analyzer (Beckman Coulter, Inc., Brea, CA, USA). For assessment of liver function, levels of total bilirubin (TBiL) and direct bilirubin (DBiL) were determined using the 7600 Fully Automated Biochemical Analyzer (Hitachi, Ltd., Tokyo, Japan).
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