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Ab70522

Manufactured by Abcam

Ab70522 is a monoclonal antibody that recognizes the CD8 antigen. CD8 is a glycoprotein expressed on the surface of cytotoxic T cells, thymocytes, and a subset of natural killer cells. This antibody can be used for the detection and characterization of CD8-positive cells in various applications.

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2 protocols using ab70522

1

Antibodies for Neurodegeneration Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-hnRNP A1 antibodies were obtained from Abcam (ab4791- rabbit polyclonal, ab5832 - mouse monoclonal) and Millipore (05-1521- mouse monoclonal; 04-1469 – mouse monoclonal). The anti-hnRNP A1 antibodies are specific for hnRNP A1-M9 and have been shown to overlap the M9 immunodominant epitope recognized by IgG isolated from MS patients [24 (link)]. Isotype-matched anti-rabbit IgGs were obtained from Abcam (ab107866) and Millipore (12-370). Antibodies were added to the culture media of SK-N-SH cells at a concentration of 8 ug/ml for each assay as determined in previous studies [12 ]. Primary antibodies utilized for protein detection by Western blot were as follows: spastin (SPG4-Abcam ab38150), paraplegin (SPG7-Abcam ab154989), spartin (SPG20-Abcam ab94950), and hnRNP A1-(Millipore 05-1521). Antibodies were used at manufacturers recommended concentration. Antibodies utilized for immunochemistry in granule studies were as follows: TAR-DNA Binding Protein (TDP-43 (Millipore MABN45)), GW-182 (Abcam ab70522), or hnRNP A2/B1 (Abcam ab6102).
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2

Antibodies for Neurodegeneration Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-hnRNP A1 antibodies were obtained from Abcam (ab4791- rabbit polyclonal, ab5832 - mouse monoclonal) and Millipore (05-1521- mouse monoclonal; 04-1469 – mouse monoclonal). The anti-hnRNP A1 antibodies are specific for hnRNP A1-M9 and have been shown to overlap the M9 immunodominant epitope recognized by IgG isolated from MS patients [24 (link)]. Isotype-matched anti-rabbit IgGs were obtained from Abcam (ab107866) and Millipore (12-370). Antibodies were added to the culture media of SK-N-SH cells at a concentration of 8 ug/ml for each assay as determined in previous studies [12 ]. Primary antibodies utilized for protein detection by Western blot were as follows: spastin (SPG4-Abcam ab38150), paraplegin (SPG7-Abcam ab154989), spartin (SPG20-Abcam ab94950), and hnRNP A1-(Millipore 05-1521). Antibodies were used at manufacturers recommended concentration. Antibodies utilized for immunochemistry in granule studies were as follows: TAR-DNA Binding Protein (TDP-43 (Millipore MABN45)), GW-182 (Abcam ab70522), or hnRNP A2/B1 (Abcam ab6102).
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