Foretinib gsk1363089

AM38, DBTRG-05MG, LN229, NMC-G1 and U87MG cells were purchased from ATCC. 8MGBA, 42MGBA were obtained from DSMZ. ATRT lines BT12, BT16 and 794 was kindly provided by Dr N. Foreman (University of Colorado). CHLA-07-BSGBM was the provided by the courtesy of Dr Anat Erdreich-Epsten (Children's Hospital Los Angeles). SF188, SF9744, SF9841 and SF9867 were obtained from the Brain Tumor Research Center (University of California, San Francisco).
Wild-type and K567RAxl in pcDNA3.1 (+) vector was kindly provided by Dr Trever Bivona (Helen Diller Cancer Center, UCSF) [30 (link)]. pcDNA3.1-v5/His plasmids containing human full-length EGFR has been described previously [32 (link)]. PLX4720 was provided by Plexxikon Inc (Berkeley, CA, USA) and HKI-272 (Neratinib) was purchased from TSZ Scientific LLC (MA, USA). Axl inhibitors foretinib (GSK1363089) and R428 (BGB324) were purchased from Selleckchem (Houston, Texas, USA) and Axon Medchem (Reston, Virginia, USA) respectively. All drugs were dissolved in dimethylsulfoxide (DMSO) at 10 mM and stored at −20°C. The final DMSO concentration in all experiment was less than 0.1% in medium.

The rabbit polyclonal anti-human Met, rabbit polyclonal anti-human phospho-Met, rabbit polyclonal anti-human Akt, rabbit polyclonal anti-human phospho-Akt, rabbit polyclonal anti-human p53, rabbit polyclonal anti-human phospho-MDM2, rabbit polyclonal anti-human PUMA and rabbit monoclonal anti-human β−actin antibodies that were used for immunoblotting and immunohistochemistry were all purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The rabbit polyclonal anti-human HGF (ab24865) used for immunoblotting and immunohistochemistry was purchased from Abcam (Cambridge, MA, USA). The rabbit polyclonal anti-human acetyl-p53 (Lys320) antibody that was used for immunoblotting and the rabbit anti-human Ki-67 antibody that was used for immunohistochemistry were purchased from Merck Millipore (Billerica, MA, USA). Foretinib (GSK1363089) was purchased from Selleck Chemicals (Houston, TX, USA). Cobalt (II) chloride (CoCl2) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The BD Falcon™ 96-well microplates that were used for the cell proliferation assays were purchased from BD (Franklin Lakes, NJ, USA).