Trimethyl psoralen tmp

Manufactured by Merck Group

Trimethyl-psoralen (TMP) is a laboratory reagent used in various research applications. It is a photosensitizing agent that can be used in combination with ultraviolet light for specific experimental procedures. The core function of TMP is to facilitate photochemical reactions in controlled laboratory settings.

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Lab products found in correlation

Complete protease inhibitors cocktail tablets, by Roche (1 mentions) Angelicin, by Merck Group (1 mentions) Hydroxyurea, by Merck Group (1 mentions) Colloidal blue staining kit, by Thermo Fisher Scientific (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Tween 20, by Thermo Fisher Scientific (1 mentions) Sds polyacrylamide bis tris gels, by Thermo Fisher Scientific (1 mentions) Ubiquitin monomer, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Psoralen

2 protocols using trimethyl psoralen tmp

Ubiquitin Topoisomers Biochemical Characterization

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Ubiquitin monomer, BSA, Tris, Triton X-100 and DTT, trimethyl-psoralen (TMP), hydroxyurea, angelicin and BrdU were purchased from Sigma-Aldrich. Di-ubiquitin topoisomers (linear, K6-, K11-, K27-, K29-, K33-, K48- and K63-linked ubiquitin–ubiquitin) were purchased from Boston Biochem. Complete protease inhibitors cocktail tablets were obtained from Roche. Tween-20, Colloidal blue staining kit and precast SDS–polyacrylamide Bis-Tris gels (Invitrogen). MMC was from Duchefa.
All recombinant proteins, plasmids and antibodies generated for the present study are available upon request and described in further detail on our reagents website (https://mrcppureagents.dundee.ac.uk/).

Perez-Oliva A.B., Lachaud C., Szyniarowski P., Muñoz I., Macartney T., Hickson I., Rouse J, & Alessi D.R. (2014). USP45 deubiquitylase controls ERCC1–XPF endonuclease-mediated DNA damage responses. The EMBO Journal, 34(3), 326-343.

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Extrachromosomal Array Integration in C. elegans

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Extrachromosal arrays were generated by injecting the aforementioned amount of DNA into the appropriate strain and selecting for the F1 progenies with the coinjection marker. Three independent lines were generated whenever possible. The integration of the extrachromosomal array was performed using UV/trimethylpsoralen (TMP) method. Briefly, late L4 young adult animals carrying the array were picked onto an NGM plate without OP50. These animals were fed trimethylpsoralen (TMP, SigmaAldrich) TMP at a final concentration of 50 μg/ml for 20 min. Then, they were UV-irradiated for 30 s at 4.5 mW/cm² (250nm peak wavelength; ~130mJ) and expanded for 3 to 4 weeks before selection. Three independent integrated lines were recovered whenever possible.

Das R., Lin L.C., Català-Castro F., Malaiwong N., Sanfeliu-Cerdán N., Porta-de-la-Riva M., Pidde A, & Krieg M. (2021). An asymmetric mechanical code ciphers curvature-dependent proprioceptor activity. Science Advances, 7(38), eabg4617.

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