3 matida

Manufactured by Bio-Techne

Sourced in United Kingdom

3-MATIDA is a laboratory instrument designed for various analytical applications. It utilizes a specialized technique to perform measurements and analyses. The core function of 3-MATIDA is to provide accurate and reliable data for research and testing purposes.

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Lab products found in correlation

4 protocols using 3 matida

Antibody and Pharmacological Agents

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Primary antibodies used in this study include a mouse antibody against mGlu1a (RRID:AB_396369; BD Biosciences, San Jose, CA), postsynaptic density protein 95 (PSD-95) (RRID:AB_2292909; UC Davis/NIH NeuroMab Facility, Davis, CA), or α-actinin (RRID:AB94325; MilliporeSigma, Billerica, MA), or a rabbit antibody against mGlu5 (RRID:AB_2295173; MilliporeSigma), calnexin (RRID:AB_2243890; Santa Cruz Biotechnology, Dallas, TX), or actin (RRID:AB_476693; MilliporeSigma). Validation data for antibodies are available from the companies. Pharmacological agents include 3-methyl-aminothiophene dicarboxylic acid (3-MATIDA, Tocris Bioscience, Minneapolis, MN), 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTEP, Tocris), and (RS)-3,5-dihydroxyphenylglycine (DHPG, Tocris). All drugs were freshly prepared at the day of experiments.

Mao L.M, & Wang J.Q. (2018). Alterations in mGlu5 receptor expression and function in the striatum in a rat depression model. Journal of neurochemistry, 145(4), 287-298.

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Neuropharmacology Receptor Antagonists

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Reagents were obtained from the following sources: (−) Nicotine hydrogen tartrate salt [nAChR agonist] (N5260, Millipore Sigma, 2013), (+)-MK-801 Maleate [NMDAR antagonist] (0924, Ascent Scientific, 2009), and APV [NMDAR antagonist] (A5282, Millipore Sigma, 2012); MLA [α7 nAChR antagonist] (1029, Tocris Biosciences, 2015), MPEP [mGluR5 antagonist] (1212, Tocris Biosciences, 2015), and 3-MATIDA [mGluR1 antagonist] (2196, Tocris Biosciences, 2015), Gabazine [GABAAR antagonist] (1262, Tocris Biosciences, 2009). Sazetidine-A (SAZ-A) tartrate [α4β2 nAChR partial agonist] was synthesized by Drs. Milton L. Brown, Mikell A. Paige, and Brian E. McDowell (Georgetown University, Washington, DC) and kindly provided by Dr. Kenneth Kellar (Georgetown University, 2015) (Xiao et al. 2006 (link)). AT-1001 [α3β4 nAChR partial agonist] was a gift from Astraea Therapeutics (Mountain View, CA, USA, 2012) (Zaveri et al. 2010 (link)).

Djemil S., Chen X., Zhang Z., Lee J., Rauf M., Pak D.T, & Dzakpasu R. (2019). Activation of nicotinic acetylcholine receptors induces potentiation and synchronization within in vitro hippocampal networks. Journal of neurochemistry, 153(4), 468-484.

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Reagents for Neuroscience Experiments

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(S)-3,5-dihydroxyphenylglycine (DHPG), (S)-(+)-α-amino-a-methylbenzeneacetic acid (LY 367385), MPEP, 3-MATIDA, WIN 55,212-2, CP 55940, AM 251, bicuculline and gabazine were purchased from Tocris (Bristol, UK). QX 314 was from Alomone laboratories (Jerusalem, Israel) and URB 602 was from Cayman Chemical (Ann Arbor, MI, USA). Tissue culture reagents were obtained from Gibco-BRL (San Giuliano Milanese, MI, Italy) and ICN Pharmaceuticals (Opera, MI, Italy). Propidium iodide (PI) was purchased from Molecular Probes (Leiden, the Netherlands). If not otherwise declared, all cell culture media were purchased from Sigma-Aldrich (Darmstadt, Germany).

Landucci E., Berlinguer-Palmini R., Baccini G., Boscia F., Gerace E., Mannaioni G, & Pellegrini-Giampietro D.E. (2022). The Neuroprotective Effects of mGlu1 Receptor Antagonists Are Mediated by an Enhancement of GABAergic Synaptic Transmission via a Presynaptic CB1 Receptor Mechanism. Cells, 11(19), 3015.

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Characterization of Pharmacological Compounds

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2-Bromo-α-ergocryptine methanesulfonate salt (BRM) was purchased from Sigma-Aldrich. Aprepitant was obtained from Selleck. All the other compounds tested were purchased from Tocris Biosciences, including SCH 23390 hydrochloride, rotigotine hydrochloride, sumanirole maleate, B-HT 920, Ro 10-5824 dihydrochloride, YM 202074, cinnabarinic acid, MTP, ICI 118,551 hydrochloride, GS 6201, PSB 1115, SC 19220, CP 154526, L-733060, CP 96345, TAM, DOX, A 412997 dihydrochloride, AMN 082 dihydrochloride, SKF 97541, Rac BHFF, SEW 2871, purmorphamine, (R)-(−)-α-methylhistamine dihydrobromide, methimepip dihydrobromide, VU 0155041 sodium salt, antalarmin hydrochloride, NBI 35965 hydrochloride, BQ 788 sodium salt, BAY36-7620, 3-MATIDA, MPEP hydrochloride, MRS 1754, SC 51322, SC 19220, and JTE 013.

Chen Y., Palczewska G., Masuho I., Gao S., Jin H., Dong Z., Gieser L., Brooks M.J., Kiser P.D., Kern T.S., Martemyanov K.A., Swaroop A, & Palczewski K. (2016). Synergistically acting agonists and antagonists of G protein–coupled receptors prevent photoreceptor cell degeneration. Science signaling, 9(438), ra74.

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