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Mouse pan dendritic cell isolation kit

Manufactured by Miltenyi Biotec
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Sourced in Germany

The Mouse Pan Dendritic Cell Isolation Kit is a laboratory tool used to isolate dendritic cells from mouse tissues. It utilizes magnetic beads coated with antibodies to selectively separate dendritic cells from other cell types in a sample.

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Lab products found in correlation

Collagenase d, by Roche (1 mentions) Histopaque 1083, by Merck Group (1 mentions)

Close spelling variants of this product

Pan Dendritic Cell Isolation Kit Cell isolation kits Isolation kits

2 protocols using mouse pan dendritic cell isolation kit

1

Isolation and Characterization of CD11c+ DCs

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CD11c+ DCs (1 × 105) were purified from lymphoid tissues of C36L1-treated mice and control vehicle (PBS) using the mouse Pan Dendritic Cell Isolation Kit according to manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Primary myeloid DCs were cultured for 48 h at 37°C, and the supernatant was collected for TGF-β quantification using the mouse-TGF-beta ELISA Set (BD, OptEIA™) detection kit according to the manufacturer’s instruction.
Figueiredo C.R., Azevedo R.A., Mousdell S., Resende-Lara P.T., Ireland L., Santos A., Girola N., Cunha R.L., Schmid M.C., Polonelli L., Travassos L.R, & Mielgo A. (2018). Blockade of MIF–CD74 Signalling on Macrophages and Dendritic Cells Restores the Antitumour Immune Response Against Metastatic Melanoma. Frontiers in Immunology, 9, 1132.
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2

Isolation of Mouse Splenic DCs

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DCs were isolated from the mouse spleen of naïve, ITD-treated, or heart transplant recipients. Briefly, spleens were digested with collagenase D (1 mg/mL; Boehringer Mannheim, Roche, Germany), and mononuclear cells were isolated by Histopaque 1083 (Sigma-Aldrich Inc., Saint Louis, MI) gradient centrifugation. Then, CD11c + cells were isolated using AUTOMACS and a mouse pan Dendritic Cell Isolation Kit (Miltenyi Biotec Inc.). Purity was >95% as determined by microscopy. Splenic CD8α + DCs and CD8α -DCs were also isolated by AUTOMACS and a mouse CD8α + Dendritic Cell Isolation Kit (Miltenyi Biotec Inc.).
Iwami D., Aramaki O., Shinohara N., Niimi M, & Shirasugi N. (2018). Administration of donor splenocytes via the respiratory tract generates CD8α+ regulatory dendritic cells and induces hyporesponsiveness to fully allogeneic cardiac grafts. Transplant immunology, 50.
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