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Sm2000

Manufactured by R&D Systems

The SM2000 is a laboratory instrument designed for performing high-throughput screening and analysis. It features a high-capacity sample tray and advanced optics for accurate and precise measurements. The core function of the SM2000 is to provide researchers and scientists with a reliable and efficient tool for conducting a wide range of analytical procedures within a laboratory setting.

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2 protocols using sm2000

1

Cytokine Profiling in Cell Culture

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Levels of IL‐2, IFN‐γ, TNF‐α, IFN‐β, and CXCL10 in cell culture supernatants were measured by ELISA kits (R&D Systems, SM2000, MIF00, MTA00B, MIFNB0, DY466‐05) according to the manufacturer's instructions.
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2

Evaluating T-cell Activation Cytokines

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Mouse splenocytes and irradiated mouse RM-1 cells were mixed and seeded in a U-bottom 96-well plate pre-coated with anti-mouse CD3 antibody overnight, and then anti-mouse CD28 antibody was added to each well. Cell supernatants from co-cultured cells were collected at 24 h (IL-2) and 72 h (IFN-γ) post-stimulation. IL-2 (R&D, SM2000) and IFN-γ (R&D, PMIF00) ELISA kits were used for measurement.
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