384-well plates were coated with Fab83 (150 nM) or equimolar amount of BSA in PBS-T overnight at 4°C. After three washes with PBS-T, plates were blocked with superblock (Thermofisher, 37515) for 2 h at RT. Next, a serial dilution of cell culture supernatant from transfected cells was incubated for 2 h at RT. After washing, goat anti-mouse IgG antibodies coupled to horseradish peroxidase (1:1000, Jackson Immuno Research, 115-035-003) were added for 1 h. Then, the plates were washed and developed with TMB (Invitrogen, SB02). The reaction was stopped with 0.5 M H2SO4 and absorbance at 450 nm was measured in a plate reader (Perkin Elmer, EnVision). The experiment was performed with technical duplicates.
Goat anti mouse igg antibodies coupled to horseradish peroxidase
Manufactured by Jackson ImmunoResearch
Goat anti-mouse IgG antibodies coupled to horseradish peroxidase is a laboratory reagent used as a detection tool in various immunoassays and immunohistochemical applications. The product consists of polyclonal goat antibodies that specifically recognize and bind to mouse immunoglobulin G (IgG) molecules, which are conjugated to the enzyme horseradish peroxidase. This conjugate can be used to detect and visualize the presence of mouse IgG in samples.
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Lab products found in correlation
2 protocols using goat anti mouse igg antibodies coupled to horseradish peroxidase
1
Fab83-Mediated Cell Supernatant ELISA
2
Antibody Screening Assay Using 384-well Plates
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384-well plates were coated with Fab83 (150 nM) or equimolar amount of BSA in PBS-T overnight at 4 °C. After three washes with PBS-T, plates were blocked with superblock (Thermofisher, 37515) for 2 h at RT. Next, a serial dilution of cell culture supernatant from transfected cells was incubated for 2 h at RT. After washing, goat anti-mouse IgG antibodies coupled to horseradish peroxidase (1:1000, Jackson Immuno Research, 115-035-003) were added for 1 h. Then, the plates were washed and developed with TMB (Invitrogen, SB02). The reaction was stopped with 0.5 M H2SO4 and absorbance at 450 nm was measured in a plate reader (Perkin Elmer, EnVision). The experiment was performed with technical duplicates.
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