MCF-7 cells, eagle’s minimum essential medium (EMEM), fetal bovine serum (FBS), Trypsin, and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from ATCC (USA). Acetonitrile, acetone, isopropanol, ethanol, alpha-cyano-4-hydroxycinnamic acid (α-CHCA), human Bcl-2 protein, anti-Bcl-2 antibody, bovine serum albumin (BSA), and bovine insulin were purchased from Sigma-Aldrich (USA). Synthetic peptide FATVVEELFR (m/z 1210.6) and isotope labeled peptide FATVVEEL(13C15N)FR (m/z 1217.6) were purchased from GenScript USA Inc. Polydimethylsiloxane (PDMS) was purchased from Dow Corning Corporation (USA). Indium tin oxide (ITO) coated glass slides (100 ohm/sq) were obtained from NANOCS (USA). Tridecafluoro-1, 1, 2, 2-tetra-trichlorosilane (TTTS) was purchased from Gelest (USA). isopropanol and acetone were from VWR (USA). SU-8 2075 photoresist and SU-8 developer were from MicroChem (USA). Trypsin for protein digestion on the MIMAS platform was purchased from Promega (USA).
Dulbecco s phosphate buffered saline
Manufactured by American Type Culture Collection
Sourced in United States
Dulbecco's phosphate-buffered saline is a balanced salt solution commonly used in cell culture and other biological applications. It maintains osmotic and pH balance, and provides a physiologically compatible environment for cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (2 mentions)
Ssoadvanced universal sybr green supermix, by Bio-Rad (2 mentions)
Dulbecco s modified eagle s medium dmem, by Avantor (2 mentions)
Heat inactivated fetal bovine serum, by Avantor (2 mentions)
Alamar blue, by Merck Group (2 mentions)
Trypsin edta solution, by American Type Culture Collection (2 mentions)
Cell culture flasks, by Santa Cruz Biotechnology (2 mentions)
Human apoptosis pcr array sab target list h96, by Bio-Rad (2 mentions)
Bovine insulin, by Merck Group (1 mentions)
Mcf 7 cell, by American Type Culture Collection (1 mentions)
Su 8 developer, by MicroChem (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Acetone, by Avantor (1 mentions)
Isopropanol, by Avantor (1 mentions)
Anti bcl 2 antibody, by Merck Group (1 mentions)
6 protocols using dulbecco s phosphate buffered saline
1
Quantitative Proteomic Analysis of Bcl-2 Protein
2
Degradation of Polycaprolactone Sutures
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PCL/8% Levo and PCL/16% Levo sutures (n = 5) were sectioned into two halves. The breaking strength of one segment was measured as described above, while the other segment was submerged in 1× Dulbecco's Phosphate Buffered Saline (ATCC, Manassas, Virginia) and shaken at 225 rpm at 37°C for 31 days. Sutures were then dried prior to measuring breaking strength.
3
Smooth Muscle Cell Culture Assays
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Lipofectamine was purchased from Invitrogen. Kits for the preparation of plasmid DNA were purchased from Qiagen. Chicken aortic smooth muscle cells (CAOSMC), growth medium, and subculturing supplies were purchased from Cell Applications, Inc. Human primary aortic smooth muscle cells (HASMC), human primary coronary artery endothelial cells (HCAEC), growth medium, Dulbecco's phosphate‐buffered saline, penicillin/streptomycin/amphotericin B, Hank's Balanced Salt Solution (HBSS), and subculturing supplies were purchased from ATCC. 3H‐2‐deoxyglucose was purchased from Perkin Elmer. The chemiluminescent secreted alkaline phosphatase (SAP) substrate disodium 3‐(4‐methoxyspiro {1,2‐dioxetane‐3,2'‐(5'‐chloro)tricyclo [3.3.1.13,7]decan}‐4‐yl)phenyl phosphate (CSPD) was purchased from CloneTech. The superoxide anion probe 2‐methyl‐6‐(4‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐A]pyrazin‐3‐one hydrochloride (MCLA) was purchased from Invitrogen. Heat‐inactivated fetal bovine serum (FBS) was purchased from Hyclone. Minimum essential medium (Eagle) (MEM) was purchased from Lonza. Collagenase and dispase were purchased from Worthington Biochemical Corporation. All other reagents were purchased from either Sigma Chemical Company or Thermo‐Fisher Scientific.
4
Apoptosis Evaluation in Cell Cultures
5
Apoptosis Evaluation in Cell Cultures
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RA (purity ≥98%) and Alamar Blue® (a solution of resazurin fluorescence dye) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trypsin-EDTA solution, penicillin/streptomycin, and Dulbecco’s phosphate-buffered saline (DPBS) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). An Annexin V-FITC Apoptosis Detection Kit Plus (cat. no. 68FT-Ann VP-S) was purchased from RayBiotech (Norcross, GA, USA). Propidium Iodide Flow Cytometry Kit (cat. No. ab139418) was purchased from Abcam (Cambridge, MA, USA). A DNA-free™ kit (cat. no. AM1907) was purchased from Life Technologies, Inc. (Thermo Fisher Scientific, Inc., Waltham, MA, USA). An iScript™ cDNA Synthesis kit (cat. no. 170–8890), SsoAdvanced™ Universal SYBR® Green Supermix, and the Human Apoptosis PCR array (SAB Target List) H96 were purchased from Bio-Rad Laboratories (Hercules, CA, USA). Dulbecco’s modified Eagle’s medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were purchased from VWR International (Radnor, PA, USA). Cell culture flasks were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Cell culture plates were purchased from Thermo USA Scientific (Ocala, FL, USA).
6
Iron Supplementation Evaluation Protocol
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FBS, Dulbecco’s phosphate buffered saline (DPBS), and Penicillin/Streptomycin (p/s) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). RPMI 1640 and IMDM culture media were purchased from Life Technologies (Grand Island, NY, USA). The 100 mM HEPES and 100 mM sodium pyruvate solutions were purchased from Fisher Scientific (Pittsburgh, PA, USA). NaOH, 2-mercaptoethanol, KMnO4, ferrozine, neocuproine, ammonium acetate, ascorbic acid, and Corning® Costar® Spin-X® centrifuge tube filters were purchased from Sigma-Aldrich (St. Louis, MO, USA). The 1000 µg/mL Iron (Fe) pure single-element standard (2% HNO3) was obtained from PerkinElmer (Waltham, MA, USA). The sodium ferric gluconate complex in sucrose injection used in this study was branded Ferrlecit® (Lot #D2C283A and Lot #D2C593A, Sanofi U.S. LLC, Bridgewater, NJ, USA) and generic SFG (Lot #132296.1, Watson Pharma Inc., Parsippany, NJ, USA). Detailed physicochemical characterizations of the Ferrlecit and generic SFG lots were reported by Sun et al. [34 ]. The concentrations were 10, 20, and 40 µg/mL, as determined by the post-approval pilot study (PER9801).
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