Haake rheowin job manager v 3

The rheological characterization of the formulations was performed in triplicate at 25 °C using a Thermo Scientific Haake RheoStress 1 rheometer (Thermo Fisher Scientific, Karlsruhe, Germany) with a cone rotor geometry C60/2-Ti (60 mm diameter, 2° angle, 0.105 mm gap between cone-plate), coupled to a thermostatic circulator (Thermo Haake Phoenix II + Haake C25P) and operated by the software the Haake Rheowin^® Job Manager v 3.3 software (Thermo Electron Corporation, Karlsruhe, Germany). Haake Rheowin^® Data manager v. 3.3 software (Thermo Electron Corporation, Karlsruhe, Germany) was used to perform the data analyses. The viscosity and flow curves were obtained under rotational runs at 25 °C for 3 min during the ramp-up period from 0 s⁻¹ to 50 s⁻¹, a subsequent 1-min period at 50 s⁻¹ (constant share rate period), and followed by a ramp-down period of 3 min from 50 s⁻¹ to 0 s⁻¹. The viscosity was determined at 50 s⁻¹ after 3 days of production. The data was fitted to different mathematical models: Newton, Casson, Ostwald, Bingham Herschel-Bulkley and Cross [29 (link)].

Rheological properties were analyzed at 25 °C ± 2 °C using a Haake Rheo Stress 1 rheometer (Thermo Fisher Scientific, Karlsruhe, Germany) equipped with a cone rotor C60/2-Ti (60 mm diameter, 2° angle, 0.105 mm gap between cone-plate). For the measurements, the device was connected to a thermostatic circulator Thermo Haake Phoenix II + Haake C25P and a computer provided with the Haake Rheowin^® Job Manager v 3.3 software (Thermo Electron Corporation, Karlsruhe, Germany) to execute the tests and Haake Rheowin^® Data manager v 3.3 software (Thermo Electron Corporation, Karlsruhe, Germany) to perform the analyses of the obtained data, as explained previously [24 (link)]. Viscosity curves and flow curves were recorded under rotational runs at 25 °C for 3 min during the ramp-up period from 0 to 50, 1 min at 50 (constant share rate period), and finally 3 min during the ramp-down period from 50 to 0. Viscosity values at 50 s⁻¹ were determined after 3 days of the production. All measurements were performed in triplicate.

The statistical analysis of the permeation studies was made using GraphPad Prism^® 6.0 (GraphPad Software Inc., San Diego, CA, USA). The values were expressed as averages ± SEM. The software packages Haake RheoWin^®Job Manager V.3.3 and RheoWin^®Data Manager V.3.3 (Thermo Electron Corporation, Karlsruhe, Germany) were used to carry out the testing and analysis of the obtained rheological data, respectively.