Reagents including, sodium hydroxide pellets, phosphate buffered saline (PBS) tablets , chloroform, magnesium chloride hexahydrate, Trizma (1 M), deoxyribonucleic acid sodium salt from calf thymus (ctDNA), 2'-deoxyadenosine monohydrate (dA), 2'- deoxycytidine hydrate (dC), thymidine (dT), 2'-deoxyguanosine hydrate (dG), DNase I (bovine pancreas), phosphodiesterase I (Crotalus adamanteus venom), alkaline phosphatase (Escherichia coli), were purchased from Sigma Aldrich (St. Louis, MO). Water, methanol, acetonitrile, ethanol, isopropanol, acetic and formic acids were purchased from Fisher Scientific (Pittsburg, PA) in the highest purity or LC-MS grade when available. The adduct (±)-anti- benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-N2- [15N5]-deoxyguanosine (15N-BaP-dG) was synthesized in our laboratory [27 ]. The activated carcinogen (±)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE) was purchased from MRI Global (Kansas City, MO). The Qiagen Blood and Cell Culture Midi® Kit and the Quant-IT™ Qubit® dsDNA BR assay kit were purchased from Invitrogen (Carlsbad, CA).
Quant it qubit dsdna br assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Quant-IT™ Qubit® dsDNA BR assay kit is a fluorescence-based method for the accurate quantitation of double-stranded DNA (dsDNA) samples. The kit utilizes a proprietary Qubit® fluorescent dye that specifically binds to dsDNA, allowing for sensitive and selective detection of DNA in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide pellet, by Merck Group (1 mentions)
Ethanol, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Alkaline phosphatase escherichia coli, by Merck Group (1 mentions)
Deoxyribonucleic acid sodium salt from, by Merck Group (1 mentions)
Qubit 3 fluorometer, by Thermo Fisher Scientific (1 mentions)
2 protocols using quant it qubit dsdna br assay kit
1
DNA Adduct Quantification Protocol
2
Quantification of DNA Concentrations
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DNA concentrations were determined using the Quant-iT Qubit dsDNA BR Assay Kit (Invitrogen, Carlsbad, CA, USA) and fluorescence was measured using the Qubit 3 Fluorometer (Invitrogen). Based on those values, the DNA was diluted to 2 ng/μL in TE (10 mM Tris-HCl (pH 8.0), 0.1 mM EDTA) (Ambion, Foster City, CA, USA) and final DNA concentrations were determined using the Quant-iT Qubit dsDNA HS Assay Kit (Invitrogen) and fluorescence measured using the Qubit 3 Fluorometer (Invitrogen).
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