Anti-TfnR (HTR-D65) monoclonal antibody was produced in hybridoma as in ref. 23. Anti-CHC (sc-12734) and anti–Dyn-2 (sc-64000) antibodies were purchased from Santa Cruz Biotechnology. FITC-conjugated anti-CD44 (G44-26) and anti-CD59 (p282-H19) monoclonal antibodies were obtained from BD Pharmingen. Horseradish peroxidase (HRP)– and AlexaFluor-conjugated antibodies were purchased from Life Technologies. Biotinylated albumin (#A8549), OPD (#P1536), nystatin (#N6261), poly-l-Lysine (#P1536), fibronectin (#F1141), laminin (#L2020), and hyaluronic acid (#H5388) were obtained from Sigma-Aldrich. Rat-tail collagen (#354236) and streptavidin-POD were purchased from BD Biosciences and from Roche, respectively. Fluoromount G and paraformaldehyde (PFA) were purchased from Electron Microscopy Sciences.
Fitc conjugated anti cd44 g44 26
Manufactured by BD
FITC-conjugated anti-CD44 (G44-26) is a laboratory reagent that consists of a fluorescein isothiocyanate (FITC) fluorescent label conjugated to an antibody that recognizes the CD44 cell surface antigen. This product is intended for use in flow cytometry and other immunoassay applications to detect and analyze cells expressing CD44.
Automatically generated - may contain errors
Lab products found in correlation
Hyaluronic acid, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Nystatin, by Merck Group (1 mentions)
Horseradish peroxidase hrp, by Thermo Fisher Scientific (1 mentions)
Rat tail collagen, by BD (1 mentions)
Anti chc sc 12734, by Santa Cruz Biotechnology (1 mentions)
Anti dyn 2 sc 64000, by Santa Cruz Biotechnology (1 mentions)
Biotinylated albumin, by Merck Group (1 mentions)
Fluoromount g, by Electron Microscopy Sciences (1 mentions)
Facslyric, by BD (1 mentions)
2 protocols using fitc conjugated anti cd44 g44 26
1
Antibody Identification and Characterization
2
Flow Cytometry Analysis of CD24/CD44 Expression
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Cells were collected with 0.05% trypsin–EDTA solution, washed, and resuspended at 5.0 × 106/mL in PBS with 2% FBS (staining medium) containing PE-conjugated anti-CD24 (ML5, BD Biosciences) and FITC-conjugated anti-CD44 (G44-26, BD Biosciences) antibodies. Cells were incubated at 4 °C for 60 min, washed with PBS, resuspended in staining medium, and analyzed using FACSLyric (BD Biosciences). Side scatter and forward scatter profiles were used to eliminate debris and cell doublets. Standard compensation was performed using single-stained cells incubated with a labeled isotype antibody. A total of 10,000 viable cells were counted.
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