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Anti vimentin ab24525

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Anti-Vimentin (ab24525) is a primary antibody that specifically binds to the vimentin protein. Vimentin is a type III intermediate filament protein found in various non-epithelial cells. This antibody can be used in applications such as immunohistochemistry, immunofluorescence, and Western blotting to detect and analyze vimentin expression.

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Lab products found in correlation

Anti wt 1 ab15249, by Abcam (2 mentions) Anti e cadherin 13 1900, by Thermo Fisher Scientific (2 mentions) Anti mesothelin 250519, by Abbiotec (2 mentions) Goat anti rabbit texas red t2767, by Thermo Fisher Scientific (2 mentions) Goat anti chicken fitc pa1 28794, by Thermo Fisher Scientific (2 mentions) Goat anti rat texas red 1010 07, by Southern Biotech (2 mentions) Stellaris rna fish probe designer, by Biosearch Technologies (1 mentions) Stellaris rna fish probe set, by Biosearch Technologies (1 mentions)

Spelling variants of this product

Vimentin (915 citations) Anti-vimentin (376 citations) Ab24525 (20 citations)

3 protocols using anti vimentin ab24525

1

Fluorescence Immunohistochemistry Protocol

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The primary antibodies used in fluorescence immunohistochemistry were obtained from commercial sources: anti-WT-1 (ab15249)(Abcam, Cambridge, UK) rabbit polyclonal primary antibody; anti-E-cadherin (13-1900)(Thermo Fisher Scientific, Waltham USA) rat monoclonal antibody; anti-Vimentin (ab24525)(Abcam) chicken polyclonal primary antibody; and anti-Mesothelin (250519)(Abbiotec, Escondido, CA USA) rabbit polyclonal antibody. The cross-absorbed detection (secondary) antibodies were also obtained from commercial sources: goat anti-rabbit Texas-red (T2767)(Thermo Fisher); goat anti-chicken FITC (PA1-28794)(Thermo Fisher); and goat anti-rat Texas-red (1010-07)(Southern Biotechnology, Birmingham AL, USA). Primary antibodies were used at a 5-fold saturating concentration. Secondary antibodies were titered to minimize nonspecific background staining; negative controls were stained with detection antibody alone.
Liu B.S., Valenzuela C.D., Mentzer K.L., Wagner W.L., Khalil H.A., Chen Z., Ackermann M, & Mentzer S.J. (2022). Topography of pleural epithelial structure enabled by en face isolation and machine learning. Journal of cellular physiology, 238(1), 274-284.
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Fluorescence Immunohistochemistry Protocol

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The primary antibodies used in fluorescence immunohistochemistry were obtained from commercial sources: anti-WT-1 (ab15249)(Abcam, Cambridge, UK) rabbit polyclonal primary antibody; anti-E-cadherin (13-1900)(Thermo Fisher Scientific, Waltham USA) rat monoclonal antibody; anti-Vimentin (ab24525)(Abcam) chicken polyclonal primary antibody; and anti-Mesothelin (250519)(Abbiotec, Escondido, CA USA) rabbit polyclonal antibody. The cross-absorbed detection (secondary) antibodies were also obtained from commercial sources: goat anti-rabbit Texas-red (T2767)(Thermo Fisher); goat anti-chicken FITC (PA1-28794)(Thermo Fisher); and goat anti-rat Texas-red (1010-07)(Southern Biotechnology, Birmingham AL, USA). Primary antibodies were used at a 5-fold saturating concentration. Secondary antibodies were titered to minimize nonspecific background staining; negative controls were stained with detection antibody alone.
Liu B.S., Valenzuela C.D., Mentzer K.L., Wagner W.L., Khalil H.A., Chen Z., Ackermann M, & Mentzer S.J. (2022). Topography of pleural epithelial structure enabled by en face isolation and machine learning. Journal of cellular physiology, 238(1), 274-284.
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3

Stellaris FISH Imaging of Retinal Slides

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Frozen Retinal Slides were obtained from Amsbio. Custom Stellaris® FISH Probes were designed against gene targets by utilising the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online at www.biosearchtech.com/stellarisdesigner (Version 3). The targets were hybridised with the Quasar 670 dye. Stellaris RNA FISH Probe set labelled with (Biosearch Technologies, Inc.), following the manufacturer’s instructions available online at www.biosearchtech.com/stellarisprotocols. The images were taken in Zeiss AxioImager Z1 (EBL) and then processed using ImageJ software (version 1.53c). For immunoFISH Anti-PKCA(H-7) Sc-8393 (Santa Cruz Biotechnology) was used at a dilution of 1:100 and Anti-Vimentin Ab24525 (Abcam) was used at a dilution of 1:500.
Gautam P., Hamashima K., Chen Y., Zeng Y., Makovoz B., Parikh B.H., Lee H.Y., Lau K.A., Su X., Wong R.C., Chan W.K., Li H., Blenkinsop T.A, & Loh Y.H. (2021). Multi-species single-cell transcriptomic analysis of ocular compartment regulons. Nature Communications, 12, 5675.
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