Microscopy was carried out using a Zeiss AxioImager M2 Imaging microscope. For bright field imaging, we used a Zeiss Axiocam 506 Color for real-color image capturing; for fluorescence images, an Axiocam 512 Monochrome camera with pseudo-color was used. Exceptionally, VGAT and KCC2 signals were captured with a Zeiss LSM800 confocal microscope. Both microscopes were used with the Zeiss ZenPro software v.2.3 (Zeiss Canada). For each staining, acquisition settings were kept consistent for all images taken.
Zenpro software v 2
Manufactured by Zeiss
Sourced in Canada
Zeiss ZenPro software v.2.3 is a comprehensive image analysis and processing platform designed for a wide range of microscopy applications. It provides advanced tools for image acquisition, visualization, and quantitative analysis.
Automatically generated - may contain errors
2 protocols using zenpro software v 2
1
Multimodal Microscopy Imaging Protocol
2
Quantifying Alzheimer's Neuropathology Markers
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Aβ (4G8 immunoreactivity) and tau neuropathology (AT8 immunoreactivity) was assessed as described in [10 (link)] at the Rush Alzheimer’s Disease Center. Z-stack Brightfield images of cortical laminae III and V from the temporal, parietal, and frontal cortices were taken with a Zeiss AxioImager M2 Imaging microscope equipped with an AxioCam 506 color digital camera (Zeiss, Canada), and Zeiss ZenPro software v.2.3 (Zeiss, Canada) was used. One representative brain section per region per case was imaged.
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