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Human erythrocyte superoxide dismutase sod

Manufactured by Merck Group

Human erythrocyte superoxide dismutase (SOD) is a lab equipment product. It is an enzyme found in red blood cells that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide.

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2 protocols using human erythrocyte superoxide dismutase sod

1

Preparation and Characterization of NPs

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Reagents for NPs (liposomes) were purchased from Avanti Polar Lipids (Alabaster, AL). Human erythrocyte superoxide dismutase (SOD) was purchased from Sigma. NHS-PEG4-Dibenzocyclooctyne (DBCO) was purchased from Click Chemistry Tools (Scottsdale, AZ). IgG was purchased from Fisher Scientific. Anti-CD31 was purchased from Biolegend. PerCP/Cy5.5-conjugated anti-CD45 antibody was purchased from BioLegend. Monoclonal antibodies – anti-mouse-VCAM-1 (clone MK2.7), anti-mouse-ICAM-1 (clone YN1/1.7.4), and anti-mouse-PECAM-1 (MEC 13.3, Biolegend) – were produced by culturing hybridoma cells, purified using protein G sepharose (GE Healthcare Bio-Sciences, Pittsburgh, PA) and dialyzed in PBS.
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2

Nanoparticle Fabrication and Antibody Conjugation

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Reagents for NPs (liposomes) were purchased from Avanti Polar Lipids (Alabaster, AL). Human erythrocyte superoxide dismutase (SOD) was purchased from Sigma. NHS-PEG4-Dibenzocyclooctyne (DBCO) was purchased from Click Chemistry Tools (Scottsdale, AZ). IgG was purchased from Fisher Scientific. Anti-CD31 was purchased from Biolegend. PerCP/Cy5.5-conjugated anti-CD45 antibody was purchased from BioLegend. Monoclonal antibodies -anti-mouse-VCAM-1 (clone MK2.7), anti-mouse-ICAM-1 (clone YN1/1.7.4), and antimouse-PECAM-1 (MEC 13.3, Biolegend) -were produced by culturing hybridoma cells, purified using protein G sepharose (GE Healthcare Bio-Sciences, Pittsburgh, PA) and dialyzed in PBS.
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