Stat3 inhibitor 6 s3i 201 sc 204304

Manufactured by Santa Cruz Biotechnology

Sourced in United States, France

STAT3 Inhibitor VI S3I-201 (sc-204304) is a potent and selective inhibitor of the STAT3 (signal transducer and activator of transcription 3) protein. STAT3 is a transcription factor that plays a crucial role in various cellular processes, including cell proliferation, survival, and differentiation. This inhibitor targets the SH2 domain of STAT3, thereby disrupting its dimerization and DNA-binding activity.

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Lab products found in correlation

Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions) β estradiol e2 e8875, by Merck Group (1 mentions) Caspase 1 colorimetric assay kit, by Abcam (1 mentions) Pi3k inhibitor wortmannin, by Merck Group (1 mentions) Phytohemagglutinin (pha), by Merck Group (1 mentions)

2 protocols using stat3 inhibitor 6 s3i 201 sc 204304

Modulating Inflammatory Signaling Pathways

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Human IL-6 Recombinant Protein (RP-8619; Invitrogen, Waltham, MA, USA) and bovine serum albumin (BSA; J10857-36; Thermo Scientific, Waltham, MA, USA) were dissolved in double-distilled water. STAT3 Inhibitor VI S3I-201 (sc-204304; Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in DMSO. Tocilizumab (TCZ; Actemra^®; Roche, Switzerland) was used and diluted in saline. β-Estradiol (E₂; E8875; Sigma, St. Louis, MO, USA) was dissolved in 100% ethanol. NF-κB inhibitor p-XSC (ab142600; Abcam, Cambridge, UK) was dissolved in DMSO. Tamoxifen (TAM/4-OHT; H6278; Sigma, St. Louis, MO, USA) was dissolved into 100% ethanol (EtOH).

Tsoi H., Man E.P., Chau K.M, & Khoo U.S. (2021). Targeting the IL-6/STAT3 Signalling Cascade to Reverse Tamoxifen Resistance in Estrogen Receptor Positive Breast Cancer. Cancers, 13(7), 1511.

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Coculture Assay for Immune-Modulatory Effects

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Cocultures were initiated by seeding ASCs in 96-well plates. MNCs were coseeded 18-24 h later, for 24-48 h, in the presence or absence of phytohemagglutinin (PHA) (5 mg/mL, Sigma-Aldrich), or anti-CD3/CD28 monoclonal antibodies (5 mg/mL; ImmunoTools, Friesoythe, Germany). Neutralizing anti-IL-6-receptor antibodies (Tocilizumab, Chugai PHArma France, Paris, France); anti-IL-1b monoclonal antibodies (R&D Systems, Minneapolis, MN); caspase inhibitor Z-YVAD-fluoromethylketone (Z-YVAD-fmk; MBL-International, Nanterre, France); PI3K inhibitor Wortmannin (Sigma-Aldrich); STAT3 Inhibitor-VI, S3I-201 (sc-204304; Santa Cruz Biotechnology); or anti-intracellular adhesion molecule (ICAM)-1 monoclonals (Beckman Coulter, Villepinte, France) were added to cocultures as indicated in the figure legends. Caspase-1 activity was measured using a caspase-1 colorimetric assay kit (BioVision, Milpitas, CA) following the manufacturer's instructions.

Eljaafari A., Robert M., Chehimi M., Chanon S., Durand C., Vial G., Bendridi N., Madec A., Disse E., Laville M., Rieusset J., Lefai E., Vidal H., & Pirola L. (2015). Adipose Tissue–Derived Stem Cells From Obese Subjects Contribute to Inflammation and Reduced Insulin Response in Adipocytes Through Differential Regulation of the Th1/Th17 Balance and Monocyte Activation. Diabetes, 64(7), 2477-2488.

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