Human HEp2 cells were incubated in a six-well plate (MatTek) and allowed to grow overnight. The cells were exposed to 10 μM of each BODIPY and incubated for 6 h (37 °C, 95% humidity, 5% CO2), followed by the addition of organelle tracers obtained from Invitrogen. The organelle tracers were used at the following concentrations: LysoSensor Green, 50 nM; MitoTracker Green, 250 nM; ER Tracker Blue/White, 100 nM; and BODIPY FL C5 Ceramide, 50 nM. The cells were incubated with the BODIPY and tracers for 30 min and washed with PBS three times before imaging. The images were acquired using a Leica DMRXA2 upright microscope with a water immersion objective and DAPI, GFP, and Texas Red filter cubes (Chroma Technologies).
Dmrxa2 upright microscope
Manufactured by Leica
The DMRXA2 is an upright microscope designed for advanced imaging and analysis applications. It features a sturdy construction and optical components that provide high-resolution and contrast performance. The DMRXA2 is capable of various microscopy techniques, including brightfield, darkfield, and polarized light microscopy.
Automatically generated - may contain errors
2 protocols using dmrxa2 upright microscope
1
Subcellular Trafficking Visualization
2
Cellular Uptake and Organelle Localization of Porphyrin Conjugates
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Human HEp2 cells were incubated in a glass
bottom 6-well plate (MatTek) and allowed to grow for 48 h. The cells
were exposed to 10 μM of each porphyrin conjugate for 6 h. Organelle
tracers were obtained from Invitrogen and used at the following concentrations:
LysoSensor Green, 50 nM; MitoTracker Green, 250 nM; ER Tracker Blue/White,
100 nM; and BODIPY FL C5 Ceramide, 1 mM. The organelle tracers were
diluted in medium, and the cells were incubated concurrently with
conjugate and tracers for 30 min before washing three times with PBS
and imaging by microscopy. Images were acquired using a Leica DM RXA2
upright microscope with 40× NA 0.8 dip objective lens and DAPI,
GFP, and Texas Red filter cubes (Chroma Technologies).
bottom 6-well plate (MatTek) and allowed to grow for 48 h. The cells
were exposed to 10 μM of each porphyrin conjugate for 6 h. Organelle
tracers were obtained from Invitrogen and used at the following concentrations:
LysoSensor Green, 50 nM; MitoTracker Green, 250 nM; ER Tracker Blue/White,
100 nM; and BODIPY FL C5 Ceramide, 1 mM. The organelle tracers were
diluted in medium, and the cells were incubated concurrently with
conjugate and tracers for 30 min before washing three times with PBS
and imaging by microscopy. Images were acquired using a Leica DM RXA2
upright microscope with 40× NA 0.8 dip objective lens and DAPI,
GFP, and Texas Red filter cubes (Chroma Technologies).
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