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3 protocols using ortho phosphoric acid 85

1

Topical Ivermectin Formulation Development

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IVM was received as a gift from Hovione PharmaScience Limited (Taipa, Macau). High Performance Liquid Chromatography (HPLC) grade Acetonitrile (Honeywell, Germany), Ethanol (Honeywell, Germany), MEthanol (Sigma-Aldrich, France) and Orthophosphoric Acid 85% (VWR, France) were used. Also, the following chemicals were used: Acetic Acid (Sigma-Aldrich, Germany), Cetostearyl Alcohol (Spectrum Chemical MFG. CORP, USA), d-Mannitol (Sigma-Aldrich, China), Liquid Paraffin (Merck KGaA, Germany), Paraffin Wax (Sigma-Aldrich, Switzerland), Phosphate Buffered Saline (PBS) Tablet (Sigma-Aldrich, USA), Poly(vinyl alcohol) (PVA) with average molecular weight of 30,000–70,000 Da (Sigma-Aldrich, Netherlands), Propylene Glycol (Sigma-Aldrich, USA), Sodium Acetate Trihydrate (Sigma-Aldrich, Germany), Sodium Dodecyl Sulfate (SDS) (Sigma-Aldrich, Japan), Tween® 60 (Sigma Life Science, USA) and Tween® 80 (Sigma-Aldrich, Germany). Soolantra 1% w/w Cream (Lot No. 0414050, Expiry Date 09/2022, Galderma, France) was used in this study as a reference comparable product.
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2

Analytical Standards for Mycotoxin Analysis

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The analytical standards for OTA, OTα, and 13C-labelled OTA as internal standard were obtained from Romer Labs (Tulln an der Donau, Austria). The 13C-labelled OTα was produced in-house by enzymatic degradation of 13C-labelled OTA into 13C-labelled OTα and phenylalanine. An analytical standard for creatinine was purchased from Merck (Darmstadt, Germany). All standards were stored as recommended by the supplier. Ortho-phosphoric acid 85%, formic acid, ACN HPLC grade, and acetic acid were obtained from VWR (Vienna, Austria). Ethyl acetate HPLC grade and ACN HPLC-MS/MS grade were purchased from Chem-Lab (Zedelgem, Belgium). Methanol (MeOH) LC-MS grade was bought from Honeywell (Charlotte, NC, USA). Sodium hydrogencarbonate and Whatman 903 protein saver cards were obtained from Merck (Darmstadt, Germany). Ultrapure water was produced in-house using a Milli-Q IQ 7000 water purification system from Merck (Darmstadt, Germany).
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3

Antioxidant Capacity Evaluation

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2,2 0 -Bipyridyl, ascorbic acid, trichloroacetic acid, DLdithiothreitol, Na 2 HPO 4 , NaH 2 PO 4 xH 2 0, N-Ethylmaleimide and citric acid monohydrate were from Sigma-Aldrich (Deisenhofen, Germany). Ortho-phosphoric acid 85%, Iron(III)chloride hexahydrate from VWR (Leuven, Belgium) and ethanol from by Fisher Scientific (Fair Lwan, NJ, USA).
Apple pur ee (brand: HIPP) and carrot pur ee (brand: POUCE) without added vitamin C were purchased in a local supermarket in Avignon/France.
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