Fruitflow was provided by By-Health Co., Ltd. (Zhuhai, Guangdong, China). Aspirin (acetylsalicylic acid) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Collagen and ADP were purchased from Chrono-Log (Havertown, PA, USA). Human platelet factor 4 (PF4; CXCL4), thromboxane B2 (TXB2), and 6-keto-prostaglandin F1α (PGF1α) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Abcam (Boston, MA, USA). Polyclonal anti-Akt antibody and monoclonal anti-Syk antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal anti-phospho-Akt (Ser473) antibody, monoclonal anti-GSK3β antibody, monoclonal anti-phospho-GSK3β (Ser9) antibody, polyclonal anti-p38 mitogen-activated protein kinase (MAPK) antibody, polyclonal anti-phospho-p38 MAPK (Thr180/Tyr182) antibody, monoclonal anti-phospho-PLCγ2 (Tyr759) antibody, and polyclonal anti- PLCγ2 antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Monoclonal anti-phospho-syk (Tyr525) were purchased from Abcam (Boston, MA, USA). Phalloidin-iFluor 555 was purchased from Abcam (Boston, MA, USA), which is one of a series of phalloidin conjugates that bind to actin filaments.
Monoclonal anti phospho akt ser473 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Monoclonal anti-phospho-Akt (Ser473) antibody is a laboratory reagent used to detect and measure the phosphorylation of Akt at serine 473. Akt is a key signaling protein involved in various cellular processes, and its phosphorylation at Ser473 is a marker of Akt activation.
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2 protocols using monoclonal anti phospho akt ser473 antibody
1
Platelet Activation Modulation by Fruitflow
2
Evaluating Tumor Angiogenesis and Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fifty-two DLBCL samples were included in tissue microarrays using duplicated cores of 1 mm per tumor sample. CD31+ microvascular density was stained and quantified as previously described.31 (link) Microvascular density values were grouped in quartiles and considered high or low when above or below the 50th percentile. Xenograft tumor samples were stained for phosphohistone H3, cleaved caspase-3 and MYC, as previously described.32 (link) Phospho-Akt was detected using a monoclonal anti-phospho-Akt-Ser473 antibody (Cell Signaling Technology). Preparations were evaluated on a DP70 or a BX51 microscope using Cell B Basic Imaging Software (Olympus).
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