Bloodstains collected from 10 patients on pieces of FTA Classic Cards (Whatman), approximately in 0.5 cm 2 in size, were incubated in the Lysis/Binding w kolumnie filtracyjnej High Pure Filter Tube (Roche Diagnostics), traktowano DNAzą oraz uzyskiwano RNA zgodnie z protokołem podanym przez producenta zestawu High Pure RNA Isolation Kit (Roche Diagnostics). RNA zawieszano w 50 µl Elution Buffer (Roche Diagnostics).
High pure filter tube
Manufactured by Roche
The High Pure Filter Tube is a laboratory equipment designed for the efficient separation and purification of various biological samples. It features a high-quality filter membrane that effectively retains target analytes while allowing the flow-through of unwanted components. The tube's compact and user-friendly design makes it a versatile tool for a wide range of laboratory applications.
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Lab products found in correlation
2 protocols using high pure filter tube
1
RNA Extraction from Bloodstains on FTA Cards
2
RNA Extraction and Purification Protocol
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Oczyszczone po traktowaniu DNAzą ekstrakty RNA oceniano spektrofotometrycznie z wykorzystaniem aparatu DS-11 (DeNovix).
Obecność zanieczyszczeń RNA w postaci DNA oceniano z wykorzystaniem zestawu Quantifiler® Duo DNA Quantification Kit (Thermo Fisher Scien-Buffer (Roche Diagnostics) at room temperature for 30 minutes. In the next step, they were placed in a filtration column (High Pure Filter Tube (Roche Diagnostics)) and treated with DNAse, producing RNA according to the protocol specified by the manufacturer of the High Pure RNA Isolation Kit (Roche Diagnostics). RNA was suspended in 50 µl of the Elution Buffer (Roche Diagnostics).
Obecność zanieczyszczeń RNA w postaci DNA oceniano z wykorzystaniem zestawu Quantifiler® Duo DNA Quantification Kit (Thermo Fisher Scien-Buffer (Roche Diagnostics) at room temperature for 30 minutes. In the next step, they were placed in a filtration column (High Pure Filter Tube (Roche Diagnostics)) and treated with DNAse, producing RNA according to the protocol specified by the manufacturer of the High Pure RNA Isolation Kit (Roche Diagnostics). RNA was suspended in 50 µl of the Elution Buffer (Roche Diagnostics).
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