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Krt14

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The KRT14 is a laboratory equipment product designed for specific research applications. It serves a core function in the analysis and investigation of targeted samples or materials. The details of its intended use or technical specifications are not provided in this response, as an unbiased and factual description cannot be given without the risk of extrapolation or interpretation.

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Lab products found in correlation

γ tubulin, by Abcam (6 mentions) Acetylated α tubulin, by Merck Group (6 mentions) Krt17, by Abcam (6 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (6 mentions) Bromodeoxyuridine (brdu), by Thermo Fisher Scientific (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (4 mentions) Ds qi1mc camera, by Nikon (4 mentions) Rpgrip1l, by OriGene (2 mentions) Phospho histone h3, by Cell Signaling Technology (2 mentions) Gorab, by Proteintech (2 mentions) Lectin helix pomatiaagglutinin hpa, by Thermo Fisher Scientific (2 mentions) Golgin 97, by Thermo Fisher Scientific (2 mentions) Deadend fluorometric tunel system, by Promega (2 mentions) Mounting medium with dapi, by Vector Laboratories (2 mentions) Ds qi1mc, by Nikon (2 mentions) Gfp yfp, by Aves Labs (2 mentions) P mek1 2, by Cell Signaling Technology (1 mentions) Vinculin, by Merck Group (1 mentions) P erk1 2, by Cell Signaling Technology (1 mentions) P pak1 2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-Krt14 (rabbit, (2 citations)

10 protocols using krt14

1

Antibody Panel for Cell Signaling

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The following antibodies were used in this study. PAK1 (Cell Signaling, 2602 for IB; Proteintech, 21401-1-AP for IHC), p-PAK1/2 (Cell Signaling, 2605 for IB; Biosynthesis biotechnology, bs-3315R for IHC), p110β (Cell Signaling, 3011), p110α (Cell Signaling, 4249), PTEN (Cell Signaling, 9552), p-AKT (Cell Signaling, 3787), AKT (Cell Signaling, 9272), p-ERK1/2 (Thr202/Tyr204) (Cell Signaling, 4370 for IB; Proteintech, 28733-1-AP for IHC), ERK (Cell Signaling, 9102), p-MEK1/2 (Cell Signaling, 9121), MEK1/2 (Cell Signaling, 9122), AR (N-20, SC-816), p-S6RP (Ser240/244) (Cell Signaling, 2215), p-S6RP (Ser235/236) (Cell Signaling, 2211), S6RP (Cell Signaling, 2217), Tubulin (Sigma-Aldrich, T5168), Vinculin (Sigma-Aldrich, clone V284), Actin (Cell Signaling, 4970), KRT5 (Covance, PRB-160P), KRT14 (Covance, PRB-155P), KRT18 (Epitomics, 1433-1), Rac1 (Millipore, clone 23A8), CD49f (BioLegend, 313611), Ep-CAM (BioLegend, 118217), Myc (9E10, Santa Cruz, sc-40), Myc-tag (9B11, Cell Signaling, 2276).
Wang H., Zhou Y., Chu C., Xiao J., Zheng S., Korpal M., Korn J.M., Penaloza T., Drake R.R., Gan W, & Gao X. (2023). Generating a Murine PTEN Null Cell Line to Discover the Key Role of p110β-PAK1 in Castration-Resistant Prostate Cancer Invasion. Molecular cancer research : MCR, 21(12).
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2

Immunochemistry of Canine Papillomavirus

Cited 1 time
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Tissues were fixed by immersion in Fekete’s acid-alcohol-formalin overnight, processed routinely, embedded in paraffin, sectioned at 6 um, and stained with hematoxylin and eosin (H&E). Serial sections were also tested for the presence of PV group-specific antigens by immunochemistry as previously described [24] (link), [26] (link) using rabbit polyclonal antibodies raised against disrupted Canis familiaris papillomavirus type 2 (CfPV2; previously designated as CPV2) virions. Antibodies reacting with the following markers were used on serial sections at concentrations as indicated on our public web page (http://tumor.informatics.jax.org/html/antibodies.html) [86] using a Ventana autostainer (Tuscon, AZ): rabbit anti-keratin 17 (KRT17) [87] (link), kindly provided by P. Coulombe, rabbit anti-mouse KRT1 (stock# PRB-165P), KRT5 (stock# PRB-160P), KRT6A (stock# PRB-169P), KRT10 (stock# PRB-159P), KRT14 (stock# PRB-155P; Covance, Berkeley, CA), Ki-67 (RM-9106-R7 pre-diluted; Thermo Fisher Scientific, Waltham, MA) and SOAT1 (1∶200; stock# ab39327, Abcam, Cambridge MA). Diaminobenzidine (Sigma, St. Louis, MO) was used as chromogen. In addition, a rabbit-anti adipophilin/ADRP (1∶200, cat #GP40, Progren, Heidelberg, Germany) was used with the avidin-biotin method and the Bluemap kit (cat #760-120, Ventana).
Sundberg J.P., Stearns T.M., Joh J., Proctor M., Ingle A., Silva K.A., Dadras S.S., Jenson A.B, & Ghim S.J. (2014). Immune Status, Strain Background, and Anatomic Site of Inoculation Affect Mouse Papillomavirus (MmuPV1) Induction of Exophytic Papillomas or Endophytic Trichoblastomas. PLoS ONE, 9(12), e113582.
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3

Immunofluorescence and TUNEL Staining Protocol

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Immunofluorescence and TUNEL staining were performed as described previously (Dai et al., 2013 (link)). The following primary antibodies were used: RPGRIP1L, 1:100 (Origene, Rockville, MD); p63, 1:100 (Santa Cruz Biotechnology, Santa Cruz, CA); KRT14, 1:1,000 (Covance, Princeton, NJ); KRT1, 1:500 (Roop et al., 1987 (link)); KRT17, 1:1,000 (Abcam, Cambridge, MA); LOR, 1:100 (Covance); NGFR, 1:100 (Promega); LEF1, 1:100 (Cell Signaling, Danvers, MA); BrdU, 1:50 (Life Technologies); acetylated α-tubulin, 1:1,000 (Sigma, St. Louis, MO); γ-tubulin, 1:1,000 (Abcam); ARL13B, 1:1,000 (#73-287, NeuroMab, Davis, CA); phospho-histone H3, 1:100 (Cell Signaling). AlexaFluor-conjugated secondary antibodies (1:250) were from Life Technologies. Sections were sealed in mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Chen J., Laclef C., Moncayo A., Snedecor E.R., Yang N., Li L., Takemaru K.I., Paus R., Schneider-Maunoury S, & Clark R.A. (2014). The ciliopathy gene Rpgrip1l is essential for hair follicle development. The Journal of investigative dermatology, 135(3), 701-709.
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4

Immunofluorescence Labeling of Tissue Specimens

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Immunofluorescence labeling of tissue specimens was performed as
described previously (Dai et al., 2013 (link)).
Cells were fixed in 4% PFA and blocked in 1% BSA prior to
incubating with primary antibodies. The following primary antibodies were used:
GORAB, 1:500 (Proteintech); KRT14, 1:1,000 (Covance); KRT1, 1:2000 (Roop et al.,
1987); KRT17, 1:200 (Abcam); LOR, 1:500 (Covance); NGFR, 1:200 (Promega); LEF1,
1:100 (Cell Signaling, Danvers, MA); acetylated α-tubulin, 1:600
(Sigma); γ-tubulin, 1:500 (Abcam); ARL13B, 1:100
(#73–287, NeuroMab); lectin Helix pomatiaagglutinin (HPA), 1:1,000 (Invitrogen); Golgin97, 1:1,000 (Molecular Probes),
and Ki67, 1:1000 (BD Pharmingen). AlexaFluor-conjugated secondary antibodies
(1:250) were from Life Technologies. Sections were sealed in mounting medium
with DAPI (Vector Laboratories). TUNEL staining were performed with DeadEnd
Fluorometric TUNEL System (Promega). Images were acquired by Nikon
80i fitted with Nikon DS-Qi1Mc camera and processed with
Photoshop 5.5 CS.
Liu Y., Snedecor E.R., Choi Y.J., Yang N., Zhang X., Xu Y., Han Y., Jones E.C., Shroyer K.R., Clark R.A., Zhang L., Qin C, & Chen J. (2015). Gorab is required for dermal condensate cells to respond to hedgehog signals during hair follicle morphogenesis. The Journal of investigative dermatology, 136(2), 378-386.
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5

Immunofluorescence Labeling Protocols for Cell Analysis

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Immunofluorescence labeling was performed as described previously 6 . The following primary antibodies were used: antibody to KRT1 was produced in the Roop lab45 (link); KRT14 (Covance; Princeton, NJ); GFP/YFP (Aves Labs Inc; Tigard, Oregon); KRT17 (Abcam, Cambridge, MA, USA); LEF1 (Cell Signaling, Danvers, MA); BrdU (Life Technologies); acetylated α-tubulin (Sigma); γ-tubulin (Abcam, Cambridge, MA), ARL13B (NeuroMab, 73-287; Davis, CA); AlexaFluor-conjugated secondary antibodies were from Life Technologies. Mounting medium with DAPI (Vector Laboratories, Burlingame, CA) was used. Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Yang N., Leung E.L., Liu C., Li L., Eguether T., Yao X.J., Jones E.C., Norris D.A., Liu A., Clark R.A., Roop D.R., Pazour G.J., Shroyer K.R, & Chen J. (2017). INTU is essential for oncogenic Hh signaling through regulating primary cilia formation in basal cell carcinoma (BCC). Oncogene, 36(35), 4997-5005.
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6

Immunofluorescence and TUNEL Staining Protocol

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Immunofluorescence and TUNEL staining were performed as described previously (Dai et al., 2013 (link)). The following primary antibodies were used: RPGRIP1L, 1:100 (Origene, Rockville, MD); p63, 1:100 (Santa Cruz Biotechnology, Santa Cruz, CA); KRT14, 1:1,000 (Covance, Princeton, NJ); KRT1, 1:500 (Roop et al., 1987 (link)); KRT17, 1:1,000 (Abcam, Cambridge, MA); LOR, 1:100 (Covance); NGFR, 1:100 (Promega); LEF1, 1:100 (Cell Signaling, Danvers, MA); BrdU, 1:50 (Life Technologies); acetylated α-tubulin, 1:1,000 (Sigma, St. Louis, MO); γ-tubulin, 1:1,000 (Abcam); ARL13B, 1:1,000 (#73-287, NeuroMab, Davis, CA); phospho-histone H3, 1:100 (Cell Signaling). AlexaFluor-conjugated secondary antibodies (1:250) were from Life Technologies. Sections were sealed in mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Chen J., Laclef C., Moncayo A., Snedecor E.R., Yang N., Li L., Takemaru K.I., Paus R., Schneider-Maunoury S, & Clark R.A. (2014). The ciliopathy gene Rpgrip1l is essential for hair follicle development. The Journal of investigative dermatology, 135(3), 701-709.
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7

Immunofluorescence Analysis of Epidermal Markers

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Ten-micrometer cryosections were washed twice in PBS containing 0.15% glycine and 0.5% bovine serum albumin (BSA) for 5 min followed by three 5 min washes in PBS. Sections were blocked with 2% BSA at room temperature followed by incubation with the primary antibody in PBS with 0.03% Tween-20 (PBST) containing 0.5% BSA overnight at 4 °C. The primary antibodies used were as follows: Krt15 (1:500, Covance; Princeton, NJ), Krt14 (1:500, Covance), Pax6 (1:300, Covance), Krt4 (1:150, Novus Biologicals, LLC; Littleton, CO), Krt10 (1:500, Covance), and Ki67 (1:100, eBioscience, Inc.; San Diego, CA). Proteins were visualized with indirect immunofluorescence with appropriate secondary antibody conjugates containing AlexaFluor® 555, AlexaFluor® 488, or AlexaFluor®647 (Life Technologies; Grand Island, NY). All samples were counterstained with 4’6-diamidino-2-phenylindole (DAPI) for nuclei and observed using a Zeiss Observer Z1 microscope (Carl Zeiss; Oberkochen, Germany).
Call M., Fischesser K., Lunn M.O, & Kao W.W. (2016). A unique lineage gives rise to the meibomian gland. Molecular Vision, 22, 168-176.
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8

Immunofluorescence Labeling of Tissue Specimens

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Immunofluorescence labeling of tissue specimens was performed as
described previously (Dai et al., 2013 (link)).
Cells were fixed in 4% PFA and blocked in 1% BSA prior to
incubating with primary antibodies. The following primary antibodies were used:
GORAB, 1:500 (Proteintech); KRT14, 1:1,000 (Covance); KRT1, 1:2000 (Roop et al.,
1987); KRT17, 1:200 (Abcam); LOR, 1:500 (Covance); NGFR, 1:200 (Promega); LEF1,
1:100 (Cell Signaling, Danvers, MA); acetylated α-tubulin, 1:600
(Sigma); γ-tubulin, 1:500 (Abcam); ARL13B, 1:100
(#73–287, NeuroMab); lectin Helix pomatiaagglutinin (HPA), 1:1,000 (Invitrogen); Golgin97, 1:1,000 (Molecular Probes),
and Ki67, 1:1000 (BD Pharmingen). AlexaFluor-conjugated secondary antibodies
(1:250) were from Life Technologies. Sections were sealed in mounting medium
with DAPI (Vector Laboratories). TUNEL staining were performed with DeadEnd
Fluorometric TUNEL System (Promega). Images were acquired by Nikon
80i fitted with Nikon DS-Qi1Mc camera and processed with
Photoshop 5.5 CS.
Liu Y., Snedecor E.R., Choi Y.J., Yang N., Zhang X., Xu Y., Han Y., Jones E.C., Shroyer K.R., Clark R.A., Zhang L., Qin C, & Chen J. (2015). Gorab is required for dermal condensate cells to respond to hedgehog signals during hair follicle morphogenesis. The Journal of investigative dermatology, 136(2), 378-386.
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9

Immunofluorescence Labeling Protocols for Cell Analysis

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Immunofluorescence labeling was performed as described previously 6 . The following primary antibodies were used: antibody to KRT1 was produced in the Roop lab45 (link); KRT14 (Covance; Princeton, NJ); GFP/YFP (Aves Labs Inc; Tigard, Oregon); KRT17 (Abcam, Cambridge, MA, USA); LEF1 (Cell Signaling, Danvers, MA); BrdU (Life Technologies); acetylated α-tubulin (Sigma); γ-tubulin (Abcam, Cambridge, MA), ARL13B (NeuroMab, 73-287; Davis, CA); AlexaFluor-conjugated secondary antibodies were from Life Technologies. Mounting medium with DAPI (Vector Laboratories, Burlingame, CA) was used. Images were acquired by Nikon 80i fitted with Nikon DS-Qi1Mc camera and processed with Photoshop 5.5 CS.
Yang N., Leung E.L., Liu C., Li L., Eguether T., Yao X.J., Jones E.C., Norris D.A., Liu A., Clark R.A., Roop D.R., Pazour G.J., Shroyer K.R, & Chen J. (2017). INTU is essential for oncogenic Hh signaling through regulating primary cilia formation in basal cell carcinoma (BCC). Oncogene, 36(35), 4997-5005.
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10

Antibody Characterization for Integrin Studies

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The antibodies used in this study are LEAF™ purified Anti-mouse CD29 Armenian hamster IgG (Biolegend; HMB1-1), Armenian hamster IgG negative control (Biolegend), Itga6 (Serotec; NKI-GoH3; 1:200 in immunohistochemistry of frozen sections (IHC-Fr), cleaved Casp3 (Cell Signaling; 5A1E; 1:400 IHC-Fr), Ki67 (Abcam; ab66155; 1:300 IHC-Fr), CD45 (PTPRC) (BD Pharmingen; 30-F11; 1:250 in IHC-Fr, 1:200 in FACS), F4/80 (CiteAb; MF48020; 1:50 in IHC-Fr), Krt14 (Biosite; PRB-155P; 1:600 in IHC-Fr), Tnf (Abcam; ab6671; 1:200 in IHC-Fr), CD31 (PECAM1) (Biolegend; 1:50 in FACS), active Itgb1 (BD Pharmingen; 9EG7; 1:100 in IHC-Fr, 1:50 in FACS), total Itgb1 (CD29-Alexa Fluor 647) (Biolegend; HMβ1–1; 1:50 in FACS), CD49f (Itga6) (Biolegend; GoH3; 1:20 in FACS), SHARPIN (Proteintech; 1:1000 WB), Krt14 (Covance; 1:1000 WB), Vimentin (D21H3, Cell Signalling; 1:1000 WB), GAPDH (5G4 Mab 6C5, Hytest; 1:1000 WB), DyLight 680- or 800-conjugated IgGs (Thermo Scientific; 1:5000 WB) and AlexaFluor 488/568/647-conjugated secondary Igs (Invitrogen; 1:300 in IHC-Fr and FACS).
Peuhu E., Salomaa S.I., De Franceschi N., Potter C.S., Sundberg J.P, & Pouwels J. (2017). Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice. PLoS ONE, 12(10), e0186628.
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