An HPLC-ESI-MS/MS system consisting of an HP1100 chromatograph (Agilent Inc. Technologies, Santa Clara, CA, USA) coupled with a mass spectrometer Esquire 4000 Ion Trap LC/MS (n) system (Bruker Daltonik GmbH, Bremen, Germany) were employed for analysis. For the HPLC chromatograph control, the ChemStation LC 3D Rev. A.10.02 (Agilent Technologies Inc., Santa Clara, CA, USA) software was used; for the for the spectrometer control, the esquire Control 5.2 (Bruker Daltonik GmbH, Bremen, Germany) software was used. The ionization process (nebulization) by electrospray was conducted at 3000 V, assisted by nitrogen as the nebulizer gas (50 psi and 10 L/min flow) and assisted by nitrogen as the drying gas at 365 °C. All the experiments were carried out in negative mode. A C18 column of 250 × 4.6 mm, 5 μm, and 100Å was used for the HPLC separation (Luna, Phenomenex Inc., Torrance, CA, USA). The analysis conditions using a linear gradient contained 0.1% formic acid (A) and water (B): 0–5 min, 95%–5% B; 5–25 min, 60%–40% B; 25–30 min, 40%–60% B; 30–40 min, 20%–80% B; 40–50 min, 0%–100%; 50–55, 95%–5%. The flow rate was 1.0 mL/min, using a wavelength of 270 nm.
Esquire 4000 ion trap lc ms n system
Manufactured by Bruker
Sourced in Germany
The Esquire 4000 Ion Trap LC/MS (n) system is a high-performance mass spectrometry instrument designed for liquid chromatography applications. It provides accurate mass analysis and structural information through tandem mass spectrometry (MS/MS) capabilities. The system features an ion trap mass analyzer for efficient ion trapping and detection.
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Lab products found in correlation
2 protocols using esquire 4000 ion trap lc ms n system
1
HPLC-ESI-MS/MS Analysis of Complex Samples
2
HPLC-ESI-MS/MS Analysis of Z. punctata Extract
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The Z. punctata extract was analyzed by HPLC-ESI-MS/MS to identify its constituents. Mass spectra were recorded using an Agilent 1100 (Agilent Technologies Inc., CA, USA) liquid chromatography system connected through a split to an Esquire 4000 Ion Trap LC/MS(n) system (Bruker Daltoniks, Germany). Ionization was performed at 3000 V assisted by nitrogen as nebulizing gas at 50 psi and as drying gas at 365ºC and a flow rate of 10 L/min. Negative ions were detected using full scan (m/z 20-2200) and normal resolution (scan speed 10,300 m/z/s; peak
with 0.6 FWHM/m/z). The trap parameters were set in ion charge control (ICC) using manufacturer default parameters, and maximum accumulation time of 200 ms. The mass spectrometric conditions for analysis were: electrospray needle, 4000 V; end plate offset, -500 V;
skimmer 1, 56.0 V; skimmer 2, 6.0 V; capillary exit offset, 84.6 V; capillary exit, 140.6 V.
Collision induced dissociation (CID) spectra were obtained with a fragmentation amplitude of 1.00 V (MS/MS) using helium as the collision gas and was automatically controlled through SmartFrag option. The mixture was analyzed using a MultoHigh 100 RP 18-5µ (250 x 4.6 mm) column (CS-Chromatographie Service GmbH, Langerwehe, Germany) maintained at 25 °C. The HPLC-MS analyses were performed using a linear gradient solvent system consisting of 1%
with 0.6 FWHM/m/z). The trap parameters were set in ion charge control (ICC) using manufacturer default parameters, and maximum accumulation time of 200 ms. The mass spectrometric conditions for analysis were: electrospray needle, 4000 V; end plate offset, -500 V;
skimmer 1, 56.0 V; skimmer 2, 6.0 V; capillary exit offset, 84.6 V; capillary exit, 140.6 V.
Collision induced dissociation (CID) spectra were obtained with a fragmentation amplitude of 1.00 V (MS/MS) using helium as the collision gas and was automatically controlled through SmartFrag option. The mixture was analyzed using a MultoHigh 100 RP 18-5µ (250 x 4.6 mm) column (CS-Chromatographie Service GmbH, Langerwehe, Germany) maintained at 25 °C. The HPLC-MS analyses were performed using a linear gradient solvent system consisting of 1%
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