Sandwich elisa

Manufactured by Cloud-Clone

Sourced in United States

The Sandwich ELISA is a laboratory equipment used for the detection and quantification of specific target analytes in a sample. It functions by immobilizing a capture antibody on a solid surface, which then binds to the target analyte. A detection antibody is then added, forming a 'sandwich' structure. The presence and concentration of the target analyte can be determined through a colorimetric or fluorometric readout.

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Lab products found in correlation

Quantikine sandwich elisa, by R&D Systems (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Sandwich-ELISA rat kit Sandwich ELISA kits ELISAs

3 protocols using sandwich elisa

Biomarker Measurement in ARIC Cohort

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Using EDTA plasma samples that had been collected at Visit 1 (90% of samples) or Visit 2 (10% of samples) and stored unthawed at −70°C until analysis in 2014-2015, ARIC measured plasma concentrations of MMP-3, MMP-9, IL-6, osteopontin, and PIIINP of the AAA cases and subcohort members. Visit 2 samples were used for participants whose Visit 1 samples had been exhausted.
According to manufacturers’ protocols, the ARIC laboratory measured MMP-3 and MMP-9 using a fluorokine multianalyte profiling assay (R&D Systems, Inc., Minneapolis, MN) on a Bio-plex 200 System (BioRad, Hercules, CA), IL-6 by Quantikine high-sensitivity sandwich ELISA (R&D Systems, Inc., Minneapolis, MN), osteopontin by Quantikine sandwich ELISA (R&D Systems, Inc., Minneapolis, MN), and PIIINP by sandwich ELISA (Cloud-Clone Corp., Houston, TX cat# SEA573Hu). The intra- and inter-assay coefficients of variations (CV) % for the ARIC samples are presented in Table 1. In addition, we analyzed repeatability of 58-59 blind duplicate pairs of ARIC Visit 1 samples split at the time of blood draw and stored until the lab assay of this study. The intraclass correlation coefficients for the duplicate pairs are reported in Table 1 as well.

Tang W., Yao L., Hoogeveen R.C., Alonso A., Couper D.J., Lutsey P.L., Steenson C.C., Guan W., Hunter D.W., Lederle F.A, & Folsom A.R. (2018). The associations of biomarkers of inflammation and extracellular matrix degradation with the risk of abdominal aortic aneurysm: the ARIC Study. Angiology, 70(2), 130-140.

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Quantifying IDO Levels in Cell Cultures

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The IDO concentration of the cell culture supernatant was measured using sandwich ELISA (Cloud Clone Corp, Texas, USA). All samples and reagents were brought to room temperature. One hundred microliters of the samples and standards were added to the appropriate well and incubated for 1 hour at 37°C. The liquid was removed from each well, added to 100 µL of the detection antibody, then incubated for 1 hour at 37°C. After a washing step, 100 µL of streptavidin-HRP was added and the plate was incubated for 30 minutes at 37°C. Following a second washing step, 90 µL of tetramethylbenzidine solution was added to each well and incubated for 20 minutes. Once the liquid began to turn blue, 50 µL of stop solution was added and the plate was run on the microplate reader at a wavelength of 450 nm. The IDO detection limit was 0.127 ng/mL. To assess the alteration of IDO production after PHA stimulation and to minimize the unstimulated baseline effect of T2DM, we subtracted the PHA-stimulated IDO concentration from the unstimulated one.

Kartika R., Wibowo H., Purnamasari D., Pradipta S, & Larasati R.A. (2020). Altered Indoleamine 2,3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes Mellitus. International Journal of Tryptophan Research : IJTR, 13, 1178646920978236.

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Evaluating Cartilage Metabolism in TNFα Inhibition

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To evaluate the effect of TNF-α inhibition on cartilage metabolism, the levels of biochemical markers of cartilage turnover were evaluated in serum samples collected at baseline and 15 months after initiating TNFαI therapy. Cartilage degradation was analyzed based on serum levels of C2C and COMP using commercially available ELISA kits purchased from BioVendor R&D (Brno, Czech Republic) and IBEX Pharmaceuticals, Inc. (Montreal, QC, Canada), respectively. Serum levels of cartilage synthesis marker, PIICP, were determined using a sandwich ELISA developed by Cloud-Clone Corp. (Katy, TX, USA). Testing of all samples in duplicate was completed in one day to eliminate the influence of inter-assay variation. The CVs for C2C and PIICP were <10%, they were and 4–8% for COMP.
Additionally, to assess the relative balance between type II collagen matrix synthesis and degradation, the C2C/CPII ratios were calculated.

Szeremeta A., Jura-Półtorak A., Zoń-Giebel A., Olczyk K, & Komosińska-Vassev K. (2023). Effects of Etanercept and Adalimumab on Serum Levels of Cartilage Remodeling Markers in Women with Rheumatoid Arthritis. Journal of Clinical Medicine, 12(16), 5185.

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