Takara primescript rt reagents kits

Cell culture reagents, 5-bromo-2'-deoxyuridine (BrdU), anti-BrdU antibody, DNase I and TRIzol, were purchased from Life Technologies (Carlsbad, CA, USA). GLP-1, Exendin9-39 (Ex9), isobutylmethylxanthine (IBMX), dexamethasone (Dex), 4',6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Oil Red O were acquired from Sigma Chemical (St. Louis, MO, USA). Complete Protease Inhibitor Cocktail was purchased from Roche Applied Science (Mannheim, Germany). TaKaRa PrimeScript^TM RT reagents kits and TaKaRa SYBR premix Ex Taq were acquired from TaKaRa Bio (Kyoto, Japan). Antibodies for β-catenin, (Ser³⁷/Thr⁴¹) non-phospho-β-catenin and FITC-conjugated anti-rabbit and anti-mouse IgG antibodies were purchased from Cell Signaling Transduction (Boston, MA, USA). Antibodies for Wnt4 and GAPDH were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Lifectamine2000 and siRNA control were purchased from Life Technologies (Grand Island, New York, USA). All other chemicals of analytical grade were acquired from Dingguo Bio (Shanghai, China).

Cell culture reagents, DNase I and TRIzol, were purchased from Life Technologies (Carlsbad, CA, United States). GLP-1, thapsigargin, tunicamycin, isobutylmethylxanthine (IBMX), dexamethasone (Dex), and 4′,6-diamidino-2-phenylindole (DAPI) were acquired from Sigma Chemical (St. Louis, MO, United States). Complete Protease Inhibitor Cocktail was purchased from Roche Applied Science (Mannheim, Germany). TaKaRa PrimeScript^TM RT reagents kits and TaKaRa SYBR premix Ex Taq were acquired from TaKaRa Bio (Kyoto, Japan). Antibodies for LC3, ATF-4, CHOP, phospho-AKT Ser47 (p-AKT), phospho-mTOR Ser2448 (p-mTOR), phospho-eIF2a Ser51 (p-eIF2a), phospho–PERK Thr980 (p-PERK), b-tublin, and FITC-conjugated anti-rabbit and anti-mouse IgG antibodies were purchased from Cell Signaling Transduction (Boston, MA, United States). Antibodies for GAPDH were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Lipofectamine2000 and siRNA control were purchased from Life Technologies (Grand Island, NY, United States). All other chemicals of analytical grade were acquired from Dingguo Bio (Shanghai, China).