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Mab 4e6

Manufactured by Mercodia
Sourced in Sweden

MAb-4E6 is a monoclonal antibody produced by Mercodia. It is designed for use in laboratory research and analysis.

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4 protocols using mab 4e6

1

Ox-LDL Quantification Using Capture ELISA

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Tissue Ox-LDL levels were measured using a capture rat ELISA (also known as a “sandwich” ELISA) kit, in which the wells of the microtiter plates were coated with the capture antibody mAb-4E6 (Mercodia, Sweden). Diluted supernatant samples (1:6561) were used for ELISA measurements. The optical density of the wells was read at 450 nm and the results were calculated.
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2

Biomarker Evaluation in Lipid Disorders

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Triacylglycerol (TG), total cholesterol (TC), low-density (LDL-C) and high-density (HDL-C) cholesterol concentrations were measured by routine laboratory (Laboratorio CQS, Madrid, Spain, which follows the UNE-ISO 15189:2007 directives) methods. Urea, creatinine, hepatic enzymes—glutamyl oxaloacetic transaminase (GOT), glutamic-pyruvatetransaminase (GPT), and gamma glutamyltransferase (GGT)—bilirrubin, and alkaline phosphatase (AP) were also determined. The concentration of LDLox was measured by sandwich enzyme-linked immunosorbent assay (ELISA) by using the monoclonal antibody mAb-4E6 (Mercodia AB, Uppsala, Sweden). Urinary thromboxane B2 (TBX2) and total isoprostanes were quantified by competitive ELISA (Enzo Biochem, Inc., New York, USA and Oxford Biomedical Research, Michigan, USA, respectively).
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3

Oxidized LDL and HDL Quantification

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Oxidized LDL (oxLDL) and oxidized HDL (oxHDL) were measured using commercially available ELISA kits through sandwich enzyme immunoassay techniques. OxLDL was measured through a kit from Mercodia AB (Uppsala, Sweden) with the same specific murine monoclonal antibody mAb-4E6 as in the assay described by Holvoet et al.,(25 (link)) with an inter- and intra-assay coefficient of variation of 1.1% and 2.8%, respectively. OxHDL was measured with a proprietary antibody from MyBioSource, Inc. (San Diego, CA.), with an inter- and intra-assay coefficient of variation of 2.9% and 8.8%, respectively.
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4

Cardiometabolic Risk Profiling Protocol

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During each clinical examination, participants were scheduled to arrive to the clinic between 8 and 10 am having fasted overnight. Participants were interviewed at each visit using a standard protocol to collect demographic information (age, sex, body mass index, waist-to-hip ratio, smoking status, medical history, and residential address). After resting for 5 minutes, averages of 3 readings of heart rate and right upper arm BP were obtained in a sitting position using an autonomic sphygmomanometer (HEM-7200; Omron, Japan). Concentrations of high-sensitivity CRP (C-reactive protein; hs-CRP) and lipid profiles, including apoA-I, total cholesterol, HDL-C, LDL (low-density lipoprotein) cholesterol (LDL-C), and triglycerides, were measured by automatic analyzer (Beckman AU5800; Beckman Coulter) at the clinical laboratory of Peking University First Hospital. Plasma oxidized LDL levels were analyzed using a monoclonal antibody, mAb-4E6 (Mercodia AB, Uppsala, Sweden), with an interassay coefficient of variation of 5.5% at 8.5 mU/L.
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