Caspase activity was measured using an active caspase-3 antibody (BD Biosciences, San Jose, CA, http://www.bdbiosciences.com ). In brief, cells were washed once in PBS, then fixed and permeabilized using the Cytofix/Cytoperm (BD Biosciences) for 20 min at room temperature, pelleted and washed with Perm/Wash™ buffer (BD Bioscience). Cells were subsequently stained with the FITC rabbit anti-active caspase-3 antibody (BD Bioscience). Cells were then washed and resuspended in BD Perm/Wash™ buffer before analysis by flow cytometry.
Fitc rabbit anti active caspase 3 antibody
Manufactured by BD
Sourced in United States
The FITC rabbit anti-active caspase-3 antibody is a laboratory reagent used for the detection of active caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody is conjugated with the fluorescent dye FITC (Fluorescein isothiocyanate), allowing for the visualization and quantification of active caspase-3 in cellular samples using techniques such as flow cytometry or immunofluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Perm wash buffer, by BD (2 mentions)
Active caspase 3 antibody, by BD (1 mentions)
Cytofix cytoperm, by BD (1 mentions)
Cytofix cytoperm solution, by BD (1 mentions)
Canto 2 flow cytometer, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Facs calibur becton dickinson flow cytometer, by BD (1 mentions)
Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions)
5 protocols using fitc rabbit anti active caspase 3 antibody
1
Quantifying Apoptosis via Caspase-3 Activity
2
Quantification of Platelet Caspase-3 Activation
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Stimulated platelets were resuspended in BD Cytofix/Cytoperm™ solution (BD Biosciences, Le pont de Claix – France) and incubated for 20 min on ice according to the manufacturer’s instructions. Platelets were then centrifuged 300 × g for 5 min, and the pellet was washed twice with BD Perm/Wash™ buffer. Platelets were resuspended in Perm/Wash™ buffer, stained with 20 µl of FITC rabbit anti-active Caspase-3 antibody (BD Biosciences) for 30 min at room temperature, washed once and analyzed with a CANTO II flow cytometer and BD FACSDiva™ software (BD Biosciences).
3
Caspase-3 Activity Quantification
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Caspase activity was assessed using the FITC rabbit anti-active caspase-3 antibody (BD Pharmingen). The cells were treated with SFN for 1 day. Caspase activity was detected by the FACSCalibur flow cytometer and data were analyzed using WinMDI version 2.9.
4
Telmisartan Induces Apoptosis Signaling
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After 24 h (for phospho-p38) or 48 h of treatment (for cleaved caspase 3) with 50 μM telmisartan, the cells were trypsinized, fixed, permeabilized, and then blocked with 1.5% BSA in PBS. Cells were then incubated with PE mouse anti-p38MAPK pT180/pY182 (612565, BD Biosciences, Franklin Lakes, USA) antibody or FITC rabbit anti-active caspase-3 antibody (51-68654x, BD Biosciences) for 1 h in the dark at room temperature. After washing, the cells were resuspended in PBS and analyzed using a FACS Calibur Becton Dickinson flow cytometer.
5
Cell Cycle Analysis and Apoptosis Assessment
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Treated cells were washed twice with cold PBS before fixing by 70% ethanol for at least 1 hour at 4°C. The fixed cells were washed twice with PBS, treated by 100 μl RNase (100 μg/ml) for 5 minutes, and then stained with 400 μl propidium iodide (PI) (50 μg/ml) for 30 minutes in the dark. The DNA contents were measured by FACSCalibur (Becton Dickson Instrument), and 1 × 104 cells from each sample were analyzed by using Cell Quest software (Becton Dickinson Instrument). For active caspase3 assay, cytofix/cytoperm fixation/permeabilization kit (BD Biosciences) coupled with FITC rabbit anti active Caspase-3 antibody (BD Biosciences) were used to stain the cells for flow cytometric analysis.
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