Log In Sign up
The largest database of trusted experimental protocols

Laurdan

Manufactured by Cayman Chemical
Visit Supplier
Sourced in United States

Laurdan is a fluorescent probe used in laboratory research. It functions as a polarity-sensitive dye, providing information about the local environment and dynamics of biomolecular systems.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl β cyclodextrin, by Cayman Chemical (1 mentions) Fv1000, by Olympus (1 mentions) Laurdan, by Thermo Fisher Scientific (1 mentions) Varioskan lux, by Thermo Fisher Scientific (1 mentions)

4 protocols using laurdan

1

Laurdan Staining for Lipid Raft Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laurdan staining was conducted as previously described [46 (link)]. Briefly, after 6 hours LPS treatment or PBS control, cells were stained with Laurdan (Cayman Chemical Company; Ann Arbor, MI). Briefly, 5 μM/L Laurdan was prepared in serum-free DMEM and incubated with 2×106 cells/mL for 30 minutes at 37°C. Cells were subsequently washed and fixed using 4% formaldehyde. To evaluate the effect of cholesterol depletion on lipid raft formation and verify Ct-B staining, cells were pretreated with 10 mM/L methyl-β-cyclodextrin (Cayman Chemical Company) in DMEM containing 0.1% bovine serum albumin for 3 minutes prior to Laurdan labeling. Laurdan was measured by two-photon microscopy (Olympus FV 1000, oil immersion, 60x objective) at 400–460 nm and 470–530 nm to calculate generalized polarization (GP)-values [46 (link)].
Two-photon microscopy images of Laurdan labeled cells were captured as 16-bit/channel TIFF format and were processed using Fiji/ImageJ (NIH). The mean intensities of red and green channels were measured of each whole cell by drawing an oval around the cell. GP-values were obtained by the formula, GP= (I400–600−I470–530) ÷ (I400–460+I470–530), where I represents the intensities of each region of interest for the respective channels (35).
Psaltis C.E., Fussner L.A., Yaeger M., Aloor J.J., Reece S.W., Kilburg-Basnyat B.J., Varikuti S., Luo B., Inks M., Sergin S., Schmidt C.A., Neufer P.D., Pennington E.R., Fisher-Wellman K.H., Chowdhury S.M., Fessler M.B., Fenton J.I., Anderson E.J., Shaikh S.R, & Gowdy K.M. (2023). The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling. Prostaglandins, leukotrienes, and essential fatty acids, 190, 102540.
+ Open protocol
+ Expand
2

Laurdan Fluorescence Assay for IAV

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laurdan (6-dodecanoyl-2-dimethylamino naphthalene; Cayman Chemical Company, Ann Arbor, MI, USA) was dissolved in dimethyl sulfoxide (Sigma-Aldrich Co. LLC), and 20 mM stock solution was prepared and added to IAV (1 × 109 FFU) to a final concentration of 20 μM. This mixture was incubated at 37 °C for 10 min and centrifuged at 21,600× g at 4 °C for 60 min. The supernatant was removed, and the pellet was suspended in 200 μL of PBS. IAV without Laurdan was also prepared using the same method. The viral liquid (20 μL) and sample solution (180 μL) were mixed and allowed to react for 5 min on a black 96-well microtiter plate. Fluorescence was measured using a microplate reader with excitation and emission wavelengths of 440 nm/490 nm. After subtracting the fluorescence value of the sample without Laurdan from the fluorescence value of the sample with Laurdan added, the generalized polarization (GP) was calculated using the following formula:
where I440 and I490 refer to the emission intensities at these wavelengths.
Hirama Y., Onishi S., Shibata R., Ishida H., Mori T, & Ota N. (2023). Antiviral Effect of Propylene Glycol against Envelope Viruses in Spray and Volatilized Forms. Viruses, 15(7), 1421.
+ Open protocol
+ Expand
3

Quantifying Membrane Order by Laurdan Staining

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
We analyzed differences in membrane order among cells with the fluorescent dye Laurdan. After treating various cells as described in the “Mitochondrial morphology assays” section, we stained cells (~ 70% confluency) with 5 μM Laurdan (Cayman Chemical, cat. 19706) as previously described [31 (link)] and imaged cells using two-photon microscopy (excitation 800 nm, emission 400–460 nm and 470–530 nm) [32 (link)]. We calculated general fluorescence polarization (GP) of Laurdan using custom MATLAB code as described previously [31 (link)].
Humphries B.A., Cutter A.C., Buschhaus J.M., Chen Y.C., Qyli T., Palagama D.S., Eckley S., Robison T.H., Bevoor A., Chiang B., Haley H.R., Sahoo S., Spinosa P.C., Neale D.B., Boppisetti J., Sahoo D., Ghosh P., Lahann J., Ross B.D., Yoon E., Luker K.E, & Luker G.D. (2020). Enhanced mitochondrial fission suppresses signaling and metastasis in triple-negative breast cancer. Breast Cancer Research : BCR, 22, 60.
+ Open protocol
+ Expand
4

Measuring Bacterial Membrane Fluidity with Laurdan

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laurdan (6‐Dodecanoyl‐N, N‐dymethyl2‐naphthylamine, Cayman Chemical Company) was used to assess membrane fluidity as previously described (Wenzel et al., 2018 (link)). Cells were grown in LB medium at 37°C and 220 rpm. At the desired OD600, Laurdan was added to a final concentration of 10 µM from a 1 mM working solution dissolved in dimethylformamide (DMF). After 10 min incubation at 37°C in the dark, cells were washed three times in pre‐warmed Laurdan Buffer and re‐suspended to OD600 of 0.4.
For spectroscopic measurements, Laurdan was excited at 350 nm and fluorescent emission was measured at 460 and 500 nm using a plate reader (VarioskanLUX, Thermo Fisher). After subtraction of background fluorescence, GP values were calculated by using the following formula: GP=I460‐I500/I460+I500.
van Tilburg A.Y., Warmer P., van Heel A.J., Sauer U, & Kuipers O.P. (2021). Membrane composition and organization of Bacillus subtilis 168 and its genome‐reduced derivative miniBacillus PG10. Microbial Biotechnology, 15(5), 1633-1651.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!