Skin sections were stained with H&E and the epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked, and stained with rat-anti-mouse Gr-1 mAb (1:50) followed by goat-anti-rat IgG secondary Ab (1:200, Southern Biotech) 4 (link). Slides were developed with 3-amino-9-ethylcarbazole (AEC) substrate solution (Vector Laboratories) and counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
Goat anti rat igg secondary ab
Manufactured by Southern Biotech
Goat-anti-rat IgG secondary Ab is a laboratory reagent used to detect the presence of rat immunoglobulin G (IgG) in samples. It is a polyclonal antibody produced in goats that binds to rat IgG molecules, providing a way to visualize or quantify the target protein in various immunoassays and experimental procedures.
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Lab products found in correlation
2 protocols using goat anti rat igg secondary ab
1
Epidermal Thickness and Immune Cell Analysis
2
Epidermal Thickness and Immune Cell Analysis
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Skin sections were stained with H&E and the epidermal thickness was determined by measuring the average interfollicular distance under the microscope in a blinded manner. For IHC staining, skin cryosections were fixed, blocked, and stained with rat-anti-mouse Gr-1 mAb (1:50) followed by goat-anti-rat IgG secondary Ab (1:200, Southern Biotech) 4 (link). Slides were developed with 3-amino-9-ethylcarbazole (AEC) substrate solution (Vector Laboratories) and counterstained with hematoxylin. Images were acquired at x200 magnification using Aperio ScanScope digital scanners.
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