Log In Sign up
The largest database of trusted experimental protocols

Cd8 pb

Manufactured by BioLegend
Visit Supplier
Sourced in United States

CD8-PB is a fluorochrome-conjugated antibody that specifically binds to the CD8 cell surface marker. CD8 is a glycoprotein expressed on the surface of cytotoxic T cells, a subset of T lymphocytes that play a crucial role in the adaptive immune response.

Automatically generated - may contain errors

Lab products found in correlation

Gr1 pb, by BioLegend (3 mentions) Cd11b pe cy7, by BioLegend (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Cd3 apc, by BioLegend (2 mentions) Cd11b apc cy7, by BioLegend (2 mentions) Cd4 a700, by BioLegend (2 mentions) Nk1.1 pe, by BioLegend (2 mentions) Cd3 pe cy7, by BD (1 mentions) Cd4 apc, by BioLegend (1 mentions) B220 apc, by BioLegend (1 mentions) C kit apc, by BioLegend (1 mentions) Cd19 pe cy7, by BioLegend (1 mentions) Erythrocyte lysis buffer, by Qiagen (1 mentions) Cyan adp flow cytometer, by Beckman Coulter (1 mentions) Ter119 pe cy7, by BioLegend (1 mentions) Gr1 pe cy7, by BioLegend (1 mentions) Sca1 pb, by BioLegend (1 mentions) Pd 1 pe cy7, by BD (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-human CD8-PB (3 citations) CD8 PB (clone SK1, (2 citations)

6 protocols using cd8 pb

1

Surface Marker Profiling of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface staining of BMC and spleen cells previously treated with erythrocyte lysis buffer (Qiagen) was performed with antibodies from BD Pharmingen against CD3-PE-Cy7, CD4-APC, CD8-PB, B220-APC, Ter119-PE-Cy7, CD11b-PE-Cy7, CD19-PE-Cy7, Gr-1-PE-Cy7, Gr-1-PB and Sca1-PB; cKit-APC from BioLegend (San Diego, CA, USA), and CD90.2-PE-Cy7 from eBioscience (San Diego, CA, USA) and measured using the CyanADP flow cytometer (Beckmann Coulter). Flow cytometry data were analyzed using the FlowJo 7.6 software (Tree Star, Inc., Ashland, OR, USA).
Sprissler C., Belenki D., Maurer H., Aumann K., Pfeifer D., Klein C., Müller T.A., Kissel S., Hülsdünker J., Alexandrovski J., Brummer T., Jumaa H., Duyster J, & Dierks C. (2014). Depletion of STAT5 blocks TEL–SYK-induced APMF-type leukemia with myelofibrosis and myelodysplasia in mice. Blood Cancer Journal, 4(8), e240-.
+ Open protocol
+ Expand
2

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorochrome-conjugated anti-mouse monoclonal antibodies CD3-AF700 (cat # 100216), CD4-BV785 (cat # 100453), CD8-PB (cat # 100725), CD25-BV650 (cat # 102038), GITR-PECy7 (cat # 120222), ICOS-PECy5 (cat # 107708), IFNg-PeCy7 (cat # 505826), and IL-2-PB (cat # 503820) were purchased from Biolegend. CD44-Percp-cyanine5.5 cat# 45-0441-80, CD62L-APCeFL780 (cat # 47-0621-82), Foxp3-APC (cat# 17-5773-82), Eos-eFL660 (cat # 50-5758-80), Helios-PeCy7 (cat # 25-9883-42), Aiolos-PE (cat # 12-5789-80),and bCatenin-eFL660 (cat # 50-2567-42) were purchased from Thermo Fisher Scientific. PD-1-PECy7 (cat# 25-9985-80), CXCR5-BV421 (cat # 562889), Bcl-6-PE (cat # 569522), and phospho-STAT5-PE (pY694) (cat # 612567) were procured from BD Biosciences.
Thomas R.M., Pahl M.C., Wang L., Grant S.F., Hancock W.W, & Wells A.D. (2024). Foxp3 depends on Ikaros for control of regulatory T cell gene expression and function. eLife, 12, RP91392.
+ Open protocol
+ Expand
3

Comprehensive CD4+ T-Cell Phenotypic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For phenotypic analysis of CD4+-T, the following antibodies were used: CD4 PE (Clone RPA-T4), IFN-γ FITC (Clone B27), HLA-DR allophycocyanin (APC) (clone G46-6) (BD Pharmingen; BD Biosciences), CD3 PerCP (clone SK7) (Becton Dickinson, Franklin Lakes, NJ, USA); CD4 PB (Clone RPA-T4), CD8 PB (Clone RPA-T8), CD45RA PE/Cy7 (clone H100), CD25 PE (clone M-A251), CD127 PerCP/Cy5.5 (clone A019D5), CD185 APC/Cy7 (clone J252D4) (Biolegend); and CD54 Pacific Blue (clone HCD54), IL-17 efluor660 (eBioscience). Intracellular staining of FoxP3 was carried out with the anti-Human Foxp3 Staining Set APC (clone 236A/E7) (eBioscience). Flow cytometry was carried out on a FACS Canto II (BD Biosciences).
Lion J., Burbach M., Cross A., Poussin K., Taflin C., Kaveri S., Haziot A., Glotz D, & Mooney N. (2017). Endothelial Cell Amplification of Regulatory T Cells Is Differentially Modified by Immunosuppressors and Intravenous Immunoglobulin. Frontiers in Immunology, 8, 1761.
+ Open protocol
+ Expand
4

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood, bone marrow, BAL, and lung cells were counted using an automated cell counter (Nexcelcom Bioscience, Lawrence, MA). Cells were incubated with Fc block (anti-mouse CD16/CD32; eBioscience) in 100 μl of FACS buffer (PBS, 0.5 % EDTA) followed by the antibody panels below. Labeled cells were analyzed using BD FACSCanto RUO (BD Biosciences, San Jose, CA) and the FlowJo data analysis software (Ashland, OR). Experiments represent an average of n = 5/group with up to 3 replicates. Antibodies used included: anti-mouse CD62L-APC, ICAM1-FITC, Ly6G-FITC, Ly6G-PerCP/Cy5.5, LFA-1-PE, CD45-APC/Cy7, CD4-PE, CD8-PB, CXCR2-PerCP/Cy5.5, Gr1-PB, Ly6C-PB (BioLegend, San Diego, CA), CD11C-APC, CD11b-PECy7, CD3e PE/Cy7, CD3e-PerCP/Cy5.5, CD71-PE, F4/80-APC (eBioscience).
Manicone A.M., Gong K., Johnston L.K, & Giannandrea M. (2016). Diet-induced obesity alters myeloid cell populations in naïve and injured lung. Respiratory Research, 17, 24.
+ Open protocol
+ Expand
5

Multiparametric Flow Cytometry for Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lung cells and splenocytes were isolated as described elsewhere (Engel et al., 2015) and stained with the following anti-mouse monoclonal antibodies for 20 min at 4 • C in the dark: CD45 PerCP, CD11b APC-Cy7, NK1.1 PE, CD19 FITC, CD3 APC, CD4 A700, CD8 PB, Gr1 PE, CD11b PE-Cy7, F4/80 APC, Siglec F APC-Cy7, CD11c PB (Biolegend, San Diego, USA or BD Bioscience, Heidelberg, Germany). Cell phenotyping was performed on LSRII flow cytometer using FACS Diva software (BD Bioscience, Heidelberg, Germany) and analyzed using FlowJo software (Tree Star Inc). Leukocytes were analyzed as follows: Neutrophils in spleen and lung (CD45+/CD11b high /Gr1 high ), Lymphocytes in spleen and lung (B cells: CD45+/CD11b-/CD19+; T cells: CD45+/CD11b-/ CD3+; NK cells: CD45+/NK1.1+/CD3-; NKT cells: CD45+/NK1.1+/ CD3+), alveolar macrophages (CD45+/Gr1-/SiglecF+/CD11c+), macrophages in spleen (CD45+/Gr1-/CD11b+/CD11c-).
Jagdmann S., Berchtold D., Gutbier B., Witzenrath M., Meisel A., Meisel C, & Dames C. (2021). Efficacy and safety of intratracheal IFN-γ treatment to reverse stroke-induced susceptibility to pulmonary bacterial infections. Journal of neuroimmunology, 355.
+ Open protocol
+ Expand
6

Flow Cytometric Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell phenotyping was performed on an LSRII flow cytometer using FACSDiva software (BD Biosciences) and FlowJo software (Tree Star Inc.) with the following anti-mouse monoclonal antibodies: CD45-PE-Cy7, CD3-APC, CD4-A700, CD8-PB, NK1.1-PE, CD11b-APC-Cy7, B220-FITC, SiglecF-PE, F4/80-A700, CD11c-FITC, CD11b-PE-TR, Gr1-PB, and MHCII-APC-Cy7 (Biolegend). Fluorescence-activated cell sorter-based cell sorting was performed on a FACSAria III cell sorter (BD Biosciences).
Engel O., Akyüz L., da Costa Goncalves A.C., Winek K., Dames C., Thielke M., Herold S., Böttcher C., Priller J., Volk H.D., Dirnagl U., Meisel C, & Meisel A. (2015). Cholinergic Pathway Suppresses Pulmonary Innate Immunity Facilitating Pneumonia After Stroke. Stroke, 46(11).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!