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Dmso dimethyl sulfoxide

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DMSO (dimethyl sulfoxide) is a colorless, polar, aprotic solvent that is widely used in various applications, including as a cryoprotectant, solvent, and penetration enhancer in laboratory settings. It has a high boiling point and is miscible with water and many organic solvents. DMSO is known for its ability to dissolve a wide range of compounds and is often used in the preparation of samples for analysis or experimentation.

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Lab products found in correlation

Potassium chloride (kcl), by Thermo Fisher Scientific (2 mentions) Calcium chloride, by Thermo Fisher Scientific (1 mentions) Magnesium chloride, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions) Sodium sulfate, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Bio-Techne (1 mentions) Sch442416, by Bio-Techne (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Spectramax m3, by Molecular Devices (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide, by Bio Basic (1 mentions) Activated basic alumina, by Merck Group (1 mentions) Benzyl alcohol, by Merck Group (1 mentions) Me6tren, by Merck Group (1 mentions) Tris 2 dimethylamino ethyl amine, by Merck Group (1 mentions) Hplc plus, by Merck Group (1 mentions) Oegma, by Merck Group (1 mentions) Ethyl 2 bromo 2 methylpropionate, by Merck Group (1 mentions) Oligo ethylene glycol methyl ether methacrylate, by Merck Group (1 mentions) 2 hydroxyethyl methacrylate, by Merck Group (1 mentions) Copper 1 bromide, by Merck Group (1 mentions)

5 protocols using dmso dimethyl sulfoxide

1

Stroke Induction and A2A Antagonist Treatment

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All reagents were purchased from Sigma Aldrich (St. Louis, MO, USA) unless otherwise noted. Phosphate buffered saline was adjusted to pH 7.4 and was made with sodium chloride (NaCl, 131.25 mM), sodium phosphate (NaH2PO4, monohydrate, 10.0 mM), potassium chloride (KCl, 3.0 mM) from Fisher Scientific (Fair Lawn, NJ, USA), sodium sulfate (Na2SO4, anhydrous, 2.0 mM), calcium chloride, (CaCl2, dihydrate, 1.2 mM), magnesium chloride (MgCl2, hexahydrate, 1.2 mM) (Fisher). DMSO (dimethyl sulfoxide) was purchased from Amresco (Solon, OH, USA). Vascular occluders (VO1.5N) to induce the stroke were obtained from DOCXS biomedical (Ukiah, CA).
The A2A receptor antagonist SCH 442416 (2-(2-Furanyl)-7-[3-(4-methoxyphenyl)propyl]-7H-pyrazolo[4,3-e][1 (link),2 (link),4 (link)]triazolo[1,5-c]pyrimidin-5-amine) was purchased from Tocris Biosciences (Ellisville, MO, USA) and was dissolved in 200 μL of DMSO and was administered at 3 mg/kg, i.p.
Ganesana M, & Venton B.J. (2018). Early changes in transient adenosine during cerebral ischemia and reperfusion injury. PLoS ONE, 13(5), e0196932.
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2

Stroke Induction Protocol in Rodents

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Sodium chloride (NaCl, 131.25 mM), sodium phosphate monohydrate (NaH2PO4, 10.0 mM, catalog number S9638) were purchased from Sigma Aldrich (St. Louis, MO, USA). Potassium chloride (KCl, 3.0 mM, catalog number P217), sodium sulfate (Na2SO4, anhydrous, 2.0 mM, catalog number S421), calcium chloride, (CaCl2, dihydrate, 1.2 mM, catalog number AC349610250), magnesium chloride (MgCl2, hexahydrate, 1.2 mM, catalog number BP214–500) were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Phosphate buffered saline (PBS) at pH 7.4 was used for all in vitro measurements and was made using above mentioned chemicals. DMSO (dimethyl sulfoxide) was purchased from Amresco (Solon, OH, USA, catalog number 0231). Vascular occluders (catalog number VO1.5N) to induce the stroke were obtained from DOCXS biomedical (Ukiah, CA, USA). Bovie cauterizer kit was purchased from Fine scientific tools (Foster City, CA, USA, catalog number 18010–00).
Ganesana M, & Venton B.J. (2021). Spontaneous, transient adenosine release is not enhanced in the CA1 region of hippocampus during severe ischemia models. Journal of neurochemistry, 159(5), 887-900.
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3

Cytotoxicity Evaluation of PEI Treatments

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HeLa (5 × 10 3 cells/well) was seeded in a 96-well plate 1 day before transfection at 37C. Subsequently, cells were treated with PEI 25K-CA and PEI 25K at 37°C for 4 hours. At the end of this period, the mixture was replaced with 100 µl of growth medium, and the cells were further incubated for 24 hours. The cell medium was replaced with 100 µl of medium containing 100 µg of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Bio Basic Inc., Amherst, NY) and incubated for another 4 hours, followed by the addition of 100 µl of DMSO (dimethyl sulfoxide) (Amresco, Solon, OH). The optical density was measured using a microplate reader (SpectraMax M3, Molecular Devices, San Jose, CA) at a wavelength of 570 nm. Cytotoxicity (%) of cells was calculated relative to cells incubated with the growth medium as a control.
Weecharangsan W., Niyomtham N., Boon-ek Y., Opanasopit P., & J. L.R. (2021). Growth inhibition of cholic acid-conjugated polyethylenimine 25K/ p53-EGFP complexes on human carcinoma cells. Journal of Applied Pharmaceutical Science.
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4

ATRP of Functionalized Methacrylates

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Ethyl 2-bromo-2-methylpropionate (EBiB, 98%), 2-hydroxyethyl methacrylate (HEMA, ≥99%), oligo(ethylene glycol) methyl ether methacrylate (OEGMA, Mn = 300 g mol−1), copper(i) bromide (CuBr, 98%), benzyl alcohol (BA, ≥99%) and tris[2-(dimethylamino)ethyl]amine (Me6TREN, ≥99%) were purchased from Sigma-Aldrich. HEMA and OEGMA were purified by passing over a short column of activated basic alumina (Sigma-Aldrich) to remove the inhibitor and stored at −27 °C. Copper bromide was purified according to methods described in ref. 49 and 50 (link) and stored under argon at 4 °C. Dimethyl sulfoxide (DMSO, ≥99.5%, for HPLC) purchased from POCH S.A. (Gliwice, Poland) and water (HPLC Plus) purchased from Sigma-Aldrich were purged with argon before use. Concentrated phosphate-buffered saline (PBS, BioUltra) from Sigma-Aldrich was used after tenfold dilution with filtered, deionized water.
Kasprów M., Machnik J., Otulakowski Ł., Dworak A, & Trzebicka B. (2019). Thermoresponsive P(HEMA-co-OEGMA) copolymers: synthesis, characteristics and solution behavior. RSC Advances, 9(70), 40966-40974.
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5

Synthesis and Characterization of P(3HB-co-4HB)

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The poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (Mn = 250,000 g/mol; dispersity index Mw/Mn = 2.5; the 4HB unit content 8.8 mol %) was purchased from Tianjin Green Bio-Science (Tianjin, China); the high molecular weight poly-γ-glutamic acid (Mn = 150,000 g/mol; dispersity index Mw/Mn = 2.05) and ultra-low molecular weight poly-γ-glutamic acid (Mn = 2000 g/mol; dispersity index Mw/Mn = 1.43) were purchased from Shandong Freda Biotechnology Co., Ltd. (Shandong, China). Tetradecyltrimethylammonium bromide, 4-toluenesulfonic acid monohydrate, and [R,S]-β-butyrolactone were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Dimethyl sulfoxide (DMSO) and N,N-dimethylformamide (DMF) were purchased from POCH SA (Gliwice, Poland). Dialysis membrane Spectra/Por (MWCO 1000) was purchased from Carl Roth (Karlsruhe, Germany).
Kwiecień I., Radecka I., Kowalczuk M., Jelonek K., Orchel A, & Adamus G. (2017). The Synthesis and Structural Characterization of Graft Copolymers Composed of γ-PGA Backbone and Oligoesters Pendant Chains. Journal of the American Society for Mass Spectrometry, 28(10), 2223-2234.
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