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Luria bertani broth and agar

Manufactured by Merck Group
Sourced in United States

Luria-Bertani (LB) broth and agar are widely used culture media for the growth of bacteria. LB broth is a nutrient-rich liquid medium, while LB agar is the same formulation solidified with agar. These products provide essential nutrients and support the cultivation of a variety of bacterial species in laboratory settings.

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2 protocols using luria bertani broth and agar

1

Antimicrobial Properties of Silver Nanoparticles

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Silver nanoparticles solution (4000 µg/ mL with the particle size of 35 nm) was purchased from Pars Nano Nasb Co (Tehran, Iran) and sterilized by filtration through 0.22 µm filters before use. Peptone water, phosphate buffered saline (PBS), Luria-Bertani (LB) broth and agar, Agar-agar, and resazurin sodium were obtained from Sigma Chemical Co (St. Louis, MO., USA). All other chemicals were purchased from Merck (Darmstadt, Germany). The plant, Zataria multiflora Boiss, was purchased from local groceries. Staph. aureus ATCC 25923 and Salm. Typhimurium ATCC 14028 were obtained from the Department of Food Hygiene and Quality Control, Urmia University, Urmia, Iran.
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2

Antimicrobial Coatings from DMAMS and PFDA

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Dimethyl amino methyl styrene (DMAMS, 95%) was purchased from Acros Organics (USA). 1H,1H,2H,2H-perfluorodecyl acrylate (PFDA, 98%) and tert-butyl peroxide (TBP, 98%) were purchased from Adamas (China). The DMAMS and PFDA monomers were vacuum purified prior to use to remove volatile impurities. Silicon wafer and polyester textile were used as substrates. Silicon wafer was used as a reference substrate for coating characterization. Escherichia coli (E. coli, ATCC 25922) and methicillin-resistant Staphylococcus aureus (MRSA, ATCC BAA40) were obtained from American Type Culture Collection (ATCC, USA). Luria-Bertani (LB) broth and agar, Dulbecco’s Modified Eagle Medium (DMEM), and newborn calf serum (NCB) were obtained from Sigma-Aldrich (USA). Phosphate Buffered Saline (PBS) was purchased from Biosharp (China). The Alamar Blue Cell Viability reagent and LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells were purchased from Thermo Fisher Scientific (USA).
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