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2 protocols using sodium hydrogen sulfate

1

Synthesis of Metal-Organic Nanomaterials

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Resorcinol (99%), Formaldehyde solution (37% in water), and branched polyethylenimine (MW = 10000) were purchased from Alfa Aesar (Haverhill, MA, USA) and used without any further processing. Cobalt acetylacetonate and nickel acetylacetonate (95%) were obtained from Bean Town Chemicals (Hudson, NH, USA) and used as received. Ethanol proof 200 (VWR) and sodium hydrogen sulfate were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Quantification of Thiol-Containing Biomolecules

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5,5'-Dithio-Bis(2-nitrobenzoic acid (DTNB), CB [7] , Cys, Hcy, polyacrylamide (37.5:1), tetramethyl ethylenediamine (TEMED), sodium dodecyl sulfate (SDS), Tris-HCl (pH 8.8), hydrogen peroxide (H 2 O 2 ), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS), calcium chloride (CaCl 2 ), lead acetate, bovine serum albumin (BSA), ammonium persulfate, glycerol, sodium chloride (NaCl), imidazole, tris-(2-carboxyethyl)phosphine (TCEP), pyridoxal phosphate (PLP), terrific Broth (TB) medium, iodoacetamide, sodium borohydride (NaBH 4 ), skim milk, potassium buffer saline (PBS), Tris-buffer saline (TBS), protein ladder, sodium hydrogen sulfate (NaHSO 4 ), isopropyl β-D-1-thiogalactopyranoside (IPTG), sodium acetate, acetic acid, glutaraldehyde, and β-mercaptoethanol were purchased from Sigma-Aldrich Chemicals (USA). Secondary anti-rat IgG antibody was purchased from Cell Signaling Technology, Inc. (USA). Double-distilled water was used for all experiments.
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