Dobutamine hydrochloride

Manufactured by Bio-Techne

Sourced in Sweden, United Kingdom

Dobutamine hydrochloride is a synthetic catecholamine used as a pharmaceutical drug. It is a positive inotropic agent, which means it increases the force of myocardial contraction. Dobutamine hydrochloride is commonly used in the treatment of heart conditions to improve cardiac output.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Bio-Techne (1 mentions) Atenolol, by Abcam (1 mentions) Verapamil hydrochloride, by Abcam (1 mentions) Digoxin, by Bio-Techne (1 mentions) 16 16 dimethyl pge2, by Bio-Techne (1 mentions) Zombie aqua viability dye, by BioLegend (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Hil 2, by Thermo Fisher Scientific (1 mentions) Ova peptide, by GenScript (1 mentions) Cgs 21680 hydrochloride, by Bio-Techne (1 mentions)

3 protocols using dobutamine hydrochloride

Pharmacological Evaluation of Compounds

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All substances and materials were purchased from Sigma‐Aldrich unless otherwise stated.
Tolterodine tartrate (Tocris), mirabegron (AdooQ Bioscience), cyclophosphamide monohydrate, pentobarbital sodium (pentobarbitone; APL, Gothenburg, Sweden), dobutamine hydrochloride (Tocris), acetyl‐β‐methylcholine chloride, N^ω‐nitro‐L‐arginine (L‐NNA), sodium chloride, potassium chloride, potassium dihydrogen phosphate, magnesium sulfate, sodium bicarbonate, D‐(+)‐glucose, calcium chloride dehydrate, and dimethyl sulfoxide (DMSO).

Patel B., Perez F., Aronsson P., Alothmani R., Carlsson T, & Winder M. (2020). Combination drug therapy against OAB normalizes micturition parameters and increases the release of nitric oxide during chemically induced cystitis. Pharmacology Research & Perspectives, 8(1), e00564.

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Preparing Cardiac Pharmacological Agents

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Isoprenaline hydrochloride, atenolol and verapamil hydrochloride were purchased from Abcam (Cambridge, UK). Dobutamine hydrochloride, digoxin and flecainide acetate were purchased from Tocris Bioscience (Oxford, UK). Stock solutions (1–10 mM) of all compounds were made and were stored at −20 °C. digoxin and dobutamine were dissolved in dimethyl sulfoxide (DMSO) to make the stock solution. The final concentration of DMSO during experimentation was less than 0.01%. Verapamil, atenolol, flecainide and isoprenaline were all dissolved in ultra-pure water to make the stock solution. All other reagents were purchased from either Sigma Aldrich (Dorset, UK) or Fisher Scientific (Loughborough, UK) unless stated otherwise.

Fletcher S., Maddock H., James R.S., Wallis R, & Gharanei M. (2020). The cardiac work-loop technique: An in vitro model for identifying and profiling drug-induced changes in inotropy using rat papillary muscles. Scientific Reports, 10, 5258.

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OT-1 T Cell Cytotoxicity Assay

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Splenocytes were isolated from OT-1 mice (4–6 weeks old) and activated with 100 nM OVA peptide (257–264; GenScript) and 50 U ml^–1 h IL-2 (PeproTech) for 48 h. After 48 h, fresh medium with 50 U ml^–1 IL-2 was added to the culture and incubated for an additional 24 h. Cells were then collected, replated and expanded at 1 × 10⁶ cells per ml.
For the specific cytotoxicity assay, tumor cells (target) were plated at 50,000 cells per well in a 24-well plate. OT-1 T cells (effector) were then added at a 1:5 target:effector ratio. Where indicated, GPCR agonists were added at the following concentrations: 1 μM 16,16-dimethyl PGE₂ (Tocris), 5 μM dobutamine hydrochloride (Tocris) and 5 μM CGS-21680 hydrochloride (Tocris). The coculture was left for 36 h, and cell viability was assessed by flow cytometric staining with Zombie Aqua viability dye (BioLegend).

, & Phillippy B.Q. (1998). Identification of Inositol 1,3,4-Trisphosphate 5-Kinase and Inositol 1,3,4,5-Tetrakisphosphate 6-Kinase in Immature Soybean Seeds. Plant Physiology, 116(1), 291-297.

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